{"id":8845,"date":"2018-03-16T09:52:55","date_gmt":"2018-03-16T02:52:55","guid":{"rendered":"http:\/\/ttud.com.vn\/?p=8845"},"modified":"2018-03-16T09:52:55","modified_gmt":"2018-03-16T02:52:55","slug":"ky-thuat-chinh-sua-gen-crispr-cua-nhat-ban-da-dat-toi-do-chinh-xac-tuyet-doi","status":"publish","type":"post","link":"https:\/\/casta.ttud.com.vn\/index.php\/ky-thuat-chinh-sua-gen-crispr-cua-nhat-ban-da-dat-toi-do-chinh-xac-tuyet-doi\/","title":{"rendered":"K\u1ef9 thu\u1eadt ch\u1ec9nh s\u1eeda gen CRISPR c\u1ee7a Nh\u1eadt B\u1ea3n \u0111\u00e3 \u0111\u1ea1t t\u1edbi \u0111\u1ed9 “ch\u00ednh x\u00e1c tuy\u1ec7t \u0111\u1ed1i”"},"content":{"rendered":"

C\u00f4ng ngh\u1ec7 ch\u1ec9nh s\u1eeda gen c\u00f3 kh\u1ea3 n\u0103ng thay \u0111\u1ed5i ho\u00e0n to\u00e0n th\u1ebf gi\u1edbi c\u1ee7a ch\u00fang ta. N\u1ed5i b\u1eadt trong s\u1ed1 c\u00e1c k\u1ef9 thu\u1eadt \u1ea5y l\u00e0\u00a0CRISPR, m\u1ed9t ph\u01b0\u01a1ng ph\u00e1p tinh ch\u1ec9nh gen gi\u00e1 r\u1ebb nh\u01b0ng ch\u00ednh x\u00e1c.<\/p>\n

CRISPR h\u1ee9a h\u1eb9n s\u1ebd s\u1eeda ch\u1eefa \u0111\u01b0\u1ee3c c\u00e1c l\u1ed7i DNA b\u1ec7nh t\u1eadt trong ch\u00fang ta, ti\u00eau di\u1ec7t c\u00e1c vi khu\u1ea9n g\u00e2y b\u1ec7nh, c\u1ea3i thi\u1ec7n dinh d\u01b0\u1ee1ng c\u1ee7a th\u1ef1c ph\u1ea9m v\u00e0 th\u1eadm ch\u00ed h\u1ed3i sinh c\u00e1c lo\u00e0i \u0111\u00e3 tuy\u1ec7t ch\u1ee7ng.<\/p>\n

Trong khi nh\u1eefng\u00a0lo ng\u1ea1i v\u1ec1 \u0111\u1ea1o \u0111\u1ee9c\u00a0c\u1ee7a c\u00f4ng ngh\u1ec7 n\u00e0y v\u1eabn c\u00f2n t\u1ed3n t\u1ea1i, v\u1ec1 m\u1eb7t k\u1ef9 thu\u1eadt, CRISPR \u0111\u00e3 ph\u00e1t tri\u1ec3n t\u1edbi m\u1ee9c c\u00f3 th\u1ec3 ch\u1ec9nh s\u1eeda t\u1eebng c\u1eb7p c\u01a1 s\u1edf (A-T, G-C) tr\u00ean DNA c\u1ee7a h\u1ec7 gen ng\u01b0\u1eddi. C\u00e1c nh\u00e0 khoa h\u1ecdc Nh\u1eadt B\u1ea3n cho bi\u1ebft h\u1ecd \u0111\u00e3 ti\u1ebfn t\u1edbi tr\u00ecnh \u0111\u1ed9 n\u00e0y v\u00e0 g\u1ecdi \u0111\u00f3 l\u00e0 k\u1ef9 thu\u1eadt ch\u1ec9nh s\u1eeda gen \u201cch\u00ednh x\u00e1c tuy\u1ec7t \u0111\u1ed1i<\/i>\u201d.<\/p>\n

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Ch\u1ec9nh s\u1eeda gen CRISPR \u0111\u00e3 \u0111\u1ea1t t\u1edbi \u0111\u1ed9 “ch\u00ednh x\u00e1c tuy\u1ec7t \u0111\u1ed1i”, theo c\u00e1c nh\u00e0 khoa h\u1ecdc Nh\u1eadt B\u1ea3n<\/p>\n<\/div>\n<\/div>\n

K\u1ef9 thu\u1eadt m\u1edbi \u0111\u01b0\u1ee3c m\u00f4 t\u1ea3 trong t\u1ea1p ch\u00ed\u00a0Nature Communications. N\u00f3 \u0111\u01b0\u1ee3c g\u1ecdi t\u1eaft l\u00e0 MhAX (Micro-hologic-Assisted eXcision). Ph\u01b0\u01a1ng ph\u00e1p n\u00e0y \u0111\u01b0\u1ee3c ph\u00e1t tri\u1ec3n b\u1edfi m\u1ed9t nh\u00f3m c\u00e1c nh\u00e0 khoa h\u1ecdc \u0111ang nghi\u00ean c\u1ee9u hi\u1ec7n t\u01b0\u1ee3ng \u0111\u1ed9t bi\u1ebfn DNA \u0111\u01a1n nucleotide (SNP-single nucleotide polymorphism) g\u00e2y ra b\u1ec7nh di truy\u1ec1n.<\/p>\n

\u0110\u1ec3 ch\u1ee9ng minh m\u1ed9t \u0111\u1ed9t bi\u1ebfn DNA \u0111\u01a1n nucleotide g\u00e2y ra m\u1ed9t c\u0103n b\u1ec7nh, b\u1ea1n c\u1ea7n so s\u00e1nh nh\u1eefng t\u1ebf b\u00e0o \u201csinh \u0111\u00f4i<\/i>\u201d, c\u00f3 DNA gi\u1ed1ng h\u1ec7t nhau, ch\u1ec9 kh\u00e1c m\u1ed9t c\u1eb7p c\u01a1 s\u1edf (A-T, G-X) duy nh\u1ea5t. \u0110i\u1ec1u n\u00e0y \u0111\u1eb7c bi\u1ec7t kh\u00f3 trong \u0111i\u1ec1u ki\u1ec7n th\u1ef1c t\u1ebf, nh\u01b0ng c\u00e1c nh\u00e0 khoa h\u1ecdc \u0111\u00e3 ph\u00e1t tri\u1ec3n th\u00e0nh c\u00f4ng k\u1ef9 thu\u1eadt MhAX \u0111\u1ec3 gi\u1ea3i quy\u1ebft v\u1ea5n \u0111\u1ec1.<\/p>\n

\u0110\u1ec3 th\u1ef1c hi\u1ec7n \u0111\u01b0\u1ee3c s\u1ef1 ch\u1ec9nh s\u1eeda si\u00eau ch\u00ednh x\u00e1c tr\u00ean m\u1ed9t c\u1eb7p c\u01a1 s\u1edf, c\u00e1c nh\u00e0 khoa h\u1ecdc \u0111\u00e3 ch\u00e8n m\u1ed9t nucleotide NSP v\u00e0o b\u00ean c\u1ea1nh m\u1ed9t gen ph\u00e1t x\u1ea1 hu\u1ef3nh quang. Gen ph\u00e1t x\u1ea1 n\u00e0y gi\u00fap c\u00e1c nh\u00e0 nghi\u00ean c\u1ee9u nh\u1eadn di\u1ec7n t\u1ebf b\u00e0o \u0111\u00e3 \u0111\u01b0\u1ee3c ch\u1ec9nh s\u1eeda.<\/p>\n

Sau \u0111\u00f3, h\u1ecd thi\u1ebft k\u1ebf m\u1ed9t chu\u1ed7i DNA tr\u00f9ng l\u1eb7p \u0111\u01b0\u1ee3c g\u1ecdi l\u00e0 microhomology \u1edf m\u1ed7i b\u00ean c\u1ee7a gen hu\u1ef3nh quang, nh\u1eafm m\u1ee5c ti\u00eau c\u00e1c v\u1ecb tr\u00ed \u0111\u1ec3 CRISPR \u0111i v\u00e0o v\u00e0 c\u1eaft DNA. Ti\u1ebfp theo, m\u1ed9t h\u1ec7 th\u1ed1ng s\u1eeda ch\u1eefa DNA trung gian b\u1eb1ng vi sinh v\u1eadt (MMEJ) lo\u1ea1i b\u1ecf gen hu\u1ef3nh quang.<\/p>\n

K\u1ebft qu\u1ea3 l\u00e0 ch\u1ec9 c\u00f2n l\u1ea1i m\u1ed9t c\u1eb7p c\u01a1 s\u1edf, d\u01b0\u1edbi d\u1ea1ng m\u1ed9t SNP, c\u00f2n l\u1ea1i \u1edf v\u1ecb tr\u00ed DNA b\u1ecb c\u1eaft v\u00e0 ch\u1ec9nh s\u1eeda.<\/p>\n

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M\u00f4 t\u1ea3 k\u1ef9 thu\u1eadt MhAX trong nghi\u00ean c\u1ee9u c\u1ee7a c\u00e1c nh\u00e0 khoa h\u1ecdc Nh\u1eadt<\/p>\n<\/div>\n<\/div>\n

Vi\u1ec7c nh\u1eafm m\u1ee5c ti\u00eau v\u00e0 ch\u1ec9nh s\u1eeda gen \u0111\u1eb7c bi\u1ec7t n\u00e0y th\u1ef1c s\u1ef1 ch\u00ednh x\u00e1c. “M\u1ee5c ti\u00eau c\u1ee7a ch\u00fang t\u00f4i l\u00e0 t\u1ea1o ra c\u00e1c c\u00f4ng ngh\u1ec7 ch\u1ec9nh s\u1eeda gien gi\u00fap n\u00e2ng cao hi\u1ec3u bi\u1ebft c\u1ee7a ch\u00fang ta v\u1ec1 c\u01a1 ch\u1ebf b\u1ec7nh t\u1eadt v\u00e0 cu\u1ed1i c\u00f9ng d\u1eabn \u0111\u1ebfn c\u00e1c ph\u01b0\u01a1ng ph\u00e1p \u0111i\u1ec1u tr\u1ecb<\/i>\u201d, Ti\u1ebfn s\u0129 Knut Woltjen, ng\u01b0\u1eddi \u0111\u1ee9ng \u0111\u1ea7u cu\u1ed9c nghi\u00ean c\u1ee9u, cho bi\u1ebft.<\/p>\n

“Ch\u00fang t\u00f4i tin t\u01b0\u1edfng r\u1eb1ng MhAX s\u1ebd c\u00f3 kh\u1ea3 n\u0103ng \u1ee9ng d\u1ee5ng r\u1ed9ng r\u00e3i trong nghi\u00ean c\u1ee9u b\u1ec7nh h\u1ecdc hi\u1ec7n t\u1ea1i c\u1ee7a con ng\u01b0\u1eddi, v\u00e0 trong t\u01b0\u01a1ng lai xa h\u01a1n n\u1eefa<\/i>“.<\/p>\n

Tham kh\u1ea3o\u00a0Futurism<\/i><\/p>\n

ngu\u1ed3n Genk<\/p>\n","protected":false},"excerpt":{"rendered":"

C\u00f4ng ngh\u1ec7 ch\u1ec9nh s\u1eeda gen c\u00f3 kh\u1ea3 n\u0103ng thay \u0111\u1ed5i ho\u00e0n to\u00e0n th\u1ebf gi\u1edbi c\u1ee7a ch\u00fang ta. N\u1ed5i b\u1eadt trong s\u1ed1 c\u00e1c k\u1ef9 thu\u1eadt \u1ea5y l\u00e0\u00a0CRISPR, m\u1ed9t ph\u01b0\u01a1ng ph\u00e1p tinh ch\u1ec9nh gen gi\u00e1 r\u1ebb nh\u01b0ng ch\u00ednh x\u00e1c. CRISPR h\u1ee9a h\u1eb9n s\u1ebd s\u1eeda ch\u1eefa \u0111\u01b0\u1ee3c c\u00e1c l\u1ed7i DNA b\u1ec7nh t\u1eadt trong ch\u00fang ta, ti\u00eau di\u1ec7t c\u00e1c […]<\/p>\n","protected":false},"author":18,"featured_media":8846,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[47],"tags":[],"class_list":["post-8845","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-tin-tuc"],"_links":{"self":[{"href":"https:\/\/casta.ttud.com.vn\/index.php\/wp-json\/wp\/v2\/posts\/8845","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/casta.ttud.com.vn\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/casta.ttud.com.vn\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/casta.ttud.com.vn\/index.php\/wp-json\/wp\/v2\/users\/18"}],"replies":[{"embeddable":true,"href":"https:\/\/casta.ttud.com.vn\/index.php\/wp-json\/wp\/v2\/comments?post=8845"}],"version-history":[{"count":2,"href":"https:\/\/casta.ttud.com.vn\/index.php\/wp-json\/wp\/v2\/posts\/8845\/revisions"}],"predecessor-version":[{"id":8900,"href":"https:\/\/casta.ttud.com.vn\/index.php\/wp-json\/wp\/v2\/posts\/8845\/revisions\/8900"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/casta.ttud.com.vn\/index.php\/wp-json\/wp\/v2\/media\/8846"}],"wp:attachment":[{"href":"https:\/\/casta.ttud.com.vn\/index.php\/wp-json\/wp\/v2\/media?parent=8845"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/casta.ttud.com.vn\/index.php\/wp-json\/wp\/v2\/categories?post=8845"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/casta.ttud.com.vn\/index.php\/wp-json\/wp\/v2\/tags?post=8845"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}