{"id":6186,"date":"2017-01-05T19:51:51","date_gmt":"2017-01-05T12:51:51","guid":{"rendered":"http:\/\/ttud.com.vn\/?p=6186"},"modified":"2017-10-03T14:19:09","modified_gmt":"2017-10-03T07:19:09","slug":"phan-lap-gien-ma-hoa-nhan-to-phien-ma-osnac45-lien-quan-toi-tinh-chong-chiu-han-cua-giong-la-moc-tuyen","status":"publish","type":"post","link":"https:\/\/casta.ttud.com.vn\/index.php\/phan-lap-gien-ma-hoa-nhan-to-phien-ma-osnac45-lien-quan-toi-tinh-chong-chiu-han-cua-giong-la-moc-tuyen\/","title":{"rendered":"Ph\u00e2n l\u1eadp Gien m\u00e3 h\u00f3a nh\u00e2n t\u1ed1 phi\u00ean m\u00e3 OSNAC45 li\u00ean quan t\u1edbi t\u00ednh ch\u1ed1ng ch\u1ecbu h\u1ea1n c\u1ee7a gi\u1ed1ng l\u00e1 m\u1ed9c tuy\u1ec1n"},"content":{"rendered":"<p><strong>Khi \u0111\u1ed1i m\u1eb7t v\u1edbi c\u00e1c \u0111i\u1ec1u ki\u1ec7n m\u00f4i tr\u01b0\u1eddng b\u1ea5t l\u1ee3i, th\u1ef1c v\u1eadt kh\u1edfi \u0111\u1ed9ng m\u1ed9t chu\u1ed7i c\u00e1c \u0111\u00e1p \u1ee9ng nh\u1eb1m b\u1ea3o v\u1ec7 c\u01a1 th\u1ec3 kh\u1ecfi c\u00e1c t\u1ed5n th\u01b0\u01a1ng do m\u00f4i tr\u01b0\u1eddng g\u00e2y ra. NAC l\u00e0 h\u1ecd protein \u0111\u1eb7c tr\u01b0ng c\u1ee7a th\u1ef1c v\u1eadt c\u00f3 vai tr\u00f2 quan tr\u1ecdng trong qu\u00e1 tr\u00ecnh ph\u00e1t tri\u1ec3n v\u00e0 \u0111\u00e1p \u1ee9ng \u0111i\u1ec1u ki\u1ec7n thu\u1eadn l\u1ee3i.<\/strong><\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-6911\" src=\"http:\/\/ttud.com.vn\/wp-content\/uploads\/2017\/01\/05012017082643-thuhalua1.png\" alt=\"\" width=\"480\" height=\"360\" srcset=\"https:\/\/casta.ttud.com.vn\/wp-content\/uploads\/2017\/01\/05012017082643-thuhalua1.png 480w, https:\/\/casta.ttud.com.vn\/wp-content\/uploads\/2017\/01\/05012017082643-thuhalua1-300x225.png 300w\" sizes=\"auto, (max-width: 480px) 100vw, 480px\" \/><\/p>\n<p>Protein NAC \u0111\u00f3ng vai tr\u00f2 quan tr\u1ecdng trong vi\u1ec7c x\u00e1c \u0111\u1ecbnh m\u00f4 ph\u00e2n sinh \u0111\u1ec9nh ch\u1ed3i, bi\u1ec7t h\u00f3a c\u00e1c c\u01a1 quan r\u1ec5, hoa trong sinh tr\u01b0\u1edfng th\u1ef1c v\u1eadt, ph\u1ea3n \u1ee9ng v\u1edbi \u0111i\u1ec1u ki\u1ec7n b\u1ecb t\u1ed5n th\u01b0\u01a1ng v\u00e0 t\u00e1c nh\u00e2n g\u00e2y h\u1ea1i t\u1ea5n c\u00f4ng, t\u0103ng c\u01b0\u1eddng t\u00ednh ch\u1ecbu h\u1ea1n, m\u1eb7n v\u00e0 c\u00e1c \u0111i\u1ec1u ki\u1ec7n b\u1ea5t l\u1ee3i th\u1eddi ti\u1ebft.<\/p>\n<p>Gi\u1ed1ng l\u00faa M\u1ed9c tuy\u1ec1n do B\u1ed9 m\u00f4n B\u1ec7nh h\u1ecdc ph\u00e2n t\u1eed cung c\u1ea5p; ch\u1ee7ng vi khu\u1ea9n E.coli DH5\u03b1 \u0111\u01b0\u1ee3c mua t\u1eeb hang Fermentas (M\u1ef9). B\u1eb1ng ph\u01b0\u01a1ng ph\u00e1p RT-PCR, s\u1eed d\u1ee5ng m\u1eabu ARN t\u1ed5ng s\u1ed1 t\u00e1ch chi\u1ebft t\u1eeb c\u00e2y l\u00faa \u0111\u01b0\u1ee3c x\u1eed l\u00fd stress h\u1ea1n, \u0111\u00e3 ph\u00e2n l\u1eadp th\u00e0nh c\u00f4ng gien m\u00e3 h\u00f3a nh\u00e2n t\u1ed1 phi\u00ean m\u00e3 OSNAC45 c\u1ee7a gi\u1ed1ng l\u00faa M\u1ed9c tuy\u1ec1n. \u0110o\u1ea1n gien ph\u00e2n l\u1eadp \u0111\u00e3 \u0111\u01b0\u1ee3c nh\u00e2n d\u00f2ng th\u00e0nh c\u00f4ng gien m\u00e3 h\u00f3a nh\u00e2n t\u1ed1 phi\u00ean m\u00e3 OSNAC45 c\u1ee7a gi\u1ed1ng l\u00faa japonica \u0111\u00e3 \u0111\u01b0\u1ee3c c\u00f4ng b\u1ed1 tr\u00ean Genbank. S\u1ea3n ph\u1ea9m nh\u00e2n d\u00f2ng gien OSNAC45 s\u1ebd \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng l\u00e0m ngu\u1ed3n v\u1eadt li\u1ec7u cho nghi\u00ean c\u1ee9u t\u1ea1o gi\u1ed1ng c\u00e2y chuy\u1ec3n gien c\u00f3 kh\u1ea3 n\u1eafng ch\u1ed1ng ch\u1ecbu cao v\u1edbi c\u00e1c \u0111i\u1ec1u ki\u1ec7n stress.<\/p>\n<p style=\"text-align: right;\"><em>T\u1ea1p ch\u00ed N\u00f4ng nghi\u1ec7p v\u00e0 ph\u00e1t tri\u1ec3n n\u00f4ng th\u00f4n th\u00e1ng 10\/2016<\/em><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Khi \u0111\u1ed1i m\u1eb7t v\u1edbi c\u00e1c \u0111i\u1ec1u ki\u1ec7n m\u00f4i tr\u01b0\u1eddng b\u1ea5t l\u1ee3i, th\u1ef1c v\u1eadt kh\u1edfi \u0111\u1ed9ng m\u1ed9t chu\u1ed7i c\u00e1c \u0111\u00e1p \u1ee9ng nh\u1eb1m b\u1ea3o v\u1ec7 c\u01a1 th\u1ec3 kh\u1ecfi c\u00e1c t\u1ed5n th\u01b0\u01a1ng do m\u00f4i tr\u01b0\u1eddng g\u00e2y ra. NAC l\u00e0 h\u1ecd protein \u0111\u1eb7c tr\u01b0ng c\u1ee7a th\u1ef1c v\u1eadt c\u00f3 vai tr\u00f2 quan tr\u1ecdng trong qu\u00e1 tr\u00ecnh ph\u00e1t tri\u1ec3n v\u00e0 \u0111\u00e1p [&hellip;]<\/p>\n","protected":false},"author":17,"featured_media":6911,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[47],"tags":[],"class_list":["post-6186","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-tin-tuc"],"_links":{"self":[{"href":"https:\/\/casta.ttud.com.vn\/index.php\/wp-json\/wp\/v2\/posts\/6186","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/casta.ttud.com.vn\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/casta.ttud.com.vn\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/casta.ttud.com.vn\/index.php\/wp-json\/wp\/v2\/users\/17"}],"replies":[{"embeddable":true,"href":"https:\/\/casta.ttud.com.vn\/index.php\/wp-json\/wp\/v2\/comments?post=6186"}],"version-history":[{"count":2,"href":"https:\/\/casta.ttud.com.vn\/index.php\/wp-json\/wp\/v2\/posts\/6186\/revisions"}],"predecessor-version":[{"id":6912,"href":"https:\/\/casta.ttud.com.vn\/index.php\/wp-json\/wp\/v2\/posts\/6186\/revisions\/6912"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/casta.ttud.com.vn\/index.php\/wp-json\/wp\/v2\/media\/6911"}],"wp:attachment":[{"href":"https:\/\/casta.ttud.com.vn\/index.php\/wp-json\/wp\/v2\/media?parent=6186"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/casta.ttud.com.vn\/index.php\/wp-json\/wp\/v2\/categories?post=6186"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/casta.ttud.com.vn\/index.php\/wp-json\/wp\/v2\/tags?post=6186"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}