{"id":36261,"date":"2024-09-26T14:21:07","date_gmt":"2024-09-26T07:21:07","guid":{"rendered":"https:\/\/ttud.com.vn\/?p=36261"},"modified":"2024-09-26T14:21:13","modified_gmt":"2024-09-26T07:21:13","slug":"tcvn-13917-22023-ve-phat-hien-va-dinh-luong-thuc-vat-ngo-chuyen-gen","status":"publish","type":"post","link":"https:\/\/casta.ttud.com.vn\/index.php\/tcvn-13917-22023-ve-phat-hien-va-dinh-luong-thuc-vat-ngo-chuyen-gen\/","title":{"rendered":"TCVN 13917-2:2023 v\u1ec1 ph\u00e1t hi\u1ec7n v\u00e0 \u0111\u1ecbnh l\u01b0\u1ee3ng th\u1ef1c v\u1eadt ng\u00f4 chuy\u1ec3n gen"},"content":{"rendered":"\n

Ng\u00f4 bi\u1ebfn \u0111\u1ed5i gen hi\u1ec7n v\u1eabn c\u00f2n l\u00e0 \u0111\u1ec1 t\u00e0i tranh c\u00e3i n\u00ean vi\u1ec7c ph\u00e1t hi\u1ec7n v\u00e0 \u0111\u1ecbnh l\u01b0\u1ee3ng th\u1ef1c v\u1eadt s\u1ef1 ki\u1ec7n ng\u00f4 chuy\u1ec3n gen MIR 604 theo Ti\u00eau chu\u1ea9n qu\u1ed1c gia TCVN 13917-2:2023 l\u00e0 \u0111i\u1ec1u c\u1ea7n thi\u1ebft.<\/p>\n\n\n\n

Ng\u00f4 bi\u1ebfn \u0111\u1ed5i gen l\u00e0 m\u1ed9t s\u1ea3n ph\u1ea9m n\u00f4ng nghi\u1ec7p m\u1edbi \u0111\u01b0\u1ee3c lai t\u1ea1o. C\u00e1ch th\u1ee9c t\u1ea1o ra gi\u1ed1ng ng\u00f4 bi\u1ebfn \u0111\u1ed5i gen d\u1ef1a v\u00e0o c\u1ea5u tr\u00fac DNA. C\u00e1c gi\u1ed1ng ng\u00f4 c\u1ee5 th\u1ec3 \u0111\u00e3 \u0111\u01b0\u1ee3c bi\u1ebfn \u0111\u1ed5i gen \u0111\u1ec3 th\u1ec3 hi\u1ec7n c\u00e1c \u0111\u1eb7c \u0111i\u1ec3m mong mu\u1ed1n v\u1ec1 m\u1eb7t n\u00f4ng nghi\u1ec7p, bao g\u1ed3m kh\u1ea3 n\u0103ng kh\u00e1ng s\u00e2u b\u1ec7nh v\u00e0 thu\u1ed1c di\u1ec7t c\u1ecf. C\u00e1c gi\u1ed1ng ng\u00f4 c\u00f3 c\u1ea3 hai \u0111\u1eb7c \u0111i\u1ec3m n\u00e0y hi\u1ec7n \u0111ang \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng \u1edf nhi\u1ec1u qu\u1ed1c gia.  <\/p>\n\n\n\n

ADN (Axit deoxyribonucleic) \u0111\u01b0\u1ee3c hi\u1ec3u l\u00e0 polymer c\u1ee7a c\u00e1c deoxyribonucleotide d\u1ea1ng s\u1ee3i \u0111\u00f4i ho\u1eb7c s\u1ee3i \u0111\u01a1n l\u00e0 ch\u1ea5t mang th\u00f4ng tin di truy\u1ec1n, \u0111\u01b0\u1ee3c m\u00e3 h\u00f3a theo chu\u1ed7i c\u00e1c base (c\u00e1c h\u1ee3p ch\u1ea5t v\u00f2ng nit\u01a1 c\u00f3 trong purin ho\u1eb7c pyrimidin) v\u00e0 c\u00f3 trong nhi\u1ec5m s\u1eafc th\u1ec3 v\u00e0 v\u1eadt li\u1ec7u nhi\u1ec5m s\u1eafc th\u1ec3 c\u1ee7a c\u00e1c b\u00e0o quan. Tuy nhi\u00ean hi\u1ec7n nay c\u00e1c s\u1ea3n ph\u1ea9m ng\u00f4 bi\u1ebfn \u0111\u1ed5i gen v\u1eabn c\u00f2n nhi\u1ec1u tranh c\u00e3i do \u0111\u00f3 t\u1ea1i nhi\u1ec1u qu\u1ed1c gia \u0111\u1ec1u c\u00f3 nh\u1eefng quy \u0111\u1ecbnh v\u1ec1 ti\u00eau chu\u1ea9n ri\u00eang. <\/p>\n\n\n\n

\u1ede n\u01b0\u1edbc ta, c\u00f9ng v\u1edbi vi\u1ec7c m\u1edf r\u1ed9ng s\u1ea3n xu\u1ea5t c\u00e2y tr\u1ed3ng bi\u1ebfn \u0111\u1ed5i gen l\u00e0 vi\u1ec7c ph\u1ea3i nh\u1eadp kh\u1ea9u ng\u00f4 v\u00e0 \u0111\u1eadu t\u01b0\u01a1ng t\u1eeb c\u00e1c n\u01b0\u1edbc ph\u1ee5c v\u1ee5 cho nhu c\u1ea7u ti\u00eau th\u1ee5 trong n\u01b0\u1edbc. K\u00e9o theo \u0111\u00f3 l\u00e0 c\u00e1c lo\u1ea1i th\u1ef1c ph\u1ea9m \u0111\u01b0\u1ee3c ch\u1ebf bi\u1ebfn t\u1eeb s\u1ea3n ph\u1ea9m c\u1ee7a c\u00e2y tr\u1ed3ng bi\u1ebfn \u0111\u1ed5i gen \u0111\u00e3 xu\u1ea5t hi\u1ec7n trong nhi\u1ec1u m\u1eb7t h\u00e0ng c\u00f3 s\u1eed d\u1ee5ng ng\u00f4, \u0111\u1eadu t\u01b0\u01a1ng l\u00e0m nguy\u00ean li\u1ec7u tr\u00ean th\u1ecb tr\u01b0\u1eddng. Song song v\u1edbi ph\u00e1t tri\u1ec3n, Vi\u1ec7t Nam \u0111\u00e3 x\u00e2y d\u1ef1ng h\u00e0ng r\u00e0o ph\u00e1p l\u00fd, c\u00f3 c\u01a1 ch\u1ebf qu\u1ea3n l\u00fd gi\u00e1m s\u00e1t, c\u00f3 quy \u0111\u1ecbnh v\u1ec1 ng\u01b0\u1ee1ng d\u00e1n nh\u00e3n hay ti\u00eau chu\u1ea9n \u0111\u1ed1i v\u1edbi th\u1ef1c ph\u1ea9m c\u00f3 s\u1eed d\u1ee5ng c\u00e2y tr\u1ed3ng v\u00e0 s\u1ea3n ph\u1ea9m c\u1ee7a c\u00e2y tr\u1ed3ng bi\u1ebfn \u0111\u1ed5i gen. <\/p>\n\n\n\n

N\u00f3i t\u1edbi ti\u00eau chu\u1ea9n, hi\u1ec7n nay B\u1ed9 Khoa h\u1ecdc v\u00e0 C\u00f4ng ngh\u1ec7 \u0111\u00e3 ban h\u00e0nh Ti\u00eau chu\u1ea9n qu\u1ed1c gia TCVN 13917-2:2023 ph\u00e1t hi\u1ec7n v\u00e0 \u0111\u1ecbnh l\u01b0\u1ee3ng th\u1ef1c v\u1eadt bi\u1ebfn \u0111\u1ed5i gen – ph\u1ea7n 2 v\u1ec1 s\u1ef1 ki\u1ec7n ng\u00f4 chuy\u1ec3n gen MIR 604. Ti\u00eau chu\u1ea9n n\u00e0y \u0111\u01b0a ra c\u00e1c ph\u01b0\u01a1ng ph\u00e1p real-time PCR (ph\u1ea3n \u1ee9ng chu\u1ed7i polymerase th\u1eddi gian th\u1ef1c) \u0111\u1ec3 ph\u00e1t hi\u1ec7n v\u00e0 \u0111\u1ecbnh l\u01b0\u1ee3ng s\u1ef1 ki\u1ec7n MIR 604 (Zea mays L.) trong c\u00e2y ng\u00f4 v\u00e0 s\u1ea3n ph\u1ea9m c\u00f3 ngu\u1ed3n g\u1ed1c t\u1eeb ng\u00f4.<\/p>\n\n\n\n

V\u1ec1 nguy\u00ean t\u1eafc ti\u00eau chu\u1ea9n n\u00e0y c\u0169ng \u0111\u01b0a ra c\u00e1c ph\u01b0\u01a1ng ph\u00e1p ph\u00e1t hi\u1ec7n tr\u00ecnh t\u1ef1 ADN \u0111\u1eb7c hi\u1ec7u s\u1ef1 ki\u1ec7n ng\u00f4 chuy\u1ec3n gen MIR 604 c\u00f3 k\u00edch th\u01b0\u1edbc 76 bp b\u1eb1ng real-time PCR (PCR l\u00e0 quy tr\u00ecnh enzyme k\u1ebft h\u1ee3p khu\u1ebfch \u0111\u1ea1i in vitro c\u00e1c \u0111o\u1ea1n ADN \u0111\u1eb7c hi\u1ec7u v\u1edbi vi\u1ec7c ph\u00e1t hi\u1ec7n c\u00e1c s\u1ea3n ph\u1ea9m PCR \u0111\u1eb7c hi\u1ec7u trong qu\u00e1 tr\u00ecnh khu\u1ebfch \u0111\u1ea1i).<\/p>\n\n\n

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Vi\u1ec7c ph\u00e1t hi\u1ec7n v\u00e0 \u0111\u1ecbnh l\u01b0\u1ee3ng ng\u00f4 chuy\u1ec3n gen MIR 604 theo ti\u00eau chu\u1ea9n gi\u00fap ph\u00e1t hi\u1ec7n sinh v\u1eadt bi\u1ebfn \u0111\u1ed5i gen v\u00e0 s\u1ea3n ph\u1ea9m c\u00f3 ngu\u1ed3n g\u1ed1c bi\u1ebfn \u0111\u1ed5i gen. \u1ea2nh minh h\u1ecda<\/em><\/h2>\n\n\n\n

\u0110\u1ecbnh l\u01b0\u1ee3ng h\u00e0m l\u01b0\u1ee3ng ADN s\u1ef1 ki\u1ec7n ng\u00f4 chuy\u1ec3n gen MIR 604, s\u1eed d\u1ee5ng \u0111\u1ed3ng th\u1eddi v\u1edbi c\u00e1c m\u1ed3i v\u00e0 \u0111o\u1ea1n d\u00f2 \u0111\u1eb7c hi\u1ec7u taxon \u0111\u1ec3 khu\u1ebfch \u0111\u1ea1i trong c\u00e1c v\u1eadt li\u1ec7u ng\u00f4 chu\u1ea9n v\u00e0 m\u1eabu th\u1eed nghi\u1ec7m. C\u00e1c s\u1ea3n ph\u1ea9m s\u1eed d\u1ee5ng quy tr\u00ecnh PCR t\u00edch l\u0169y \u0111\u01b0\u1ee3c \u0111o \u1edf m\u1ed7i chu tr\u00ecnh (real-time) b\u1eb1ng \u0111o\u1ea1n d\u00f2 oligonucleotit \u0111\u1eb7c hi\u1ec7u tr\u00ecnh t\u1ef1 \u0111\u00edch \u0111\u01b0\u1ee3c \u0111\u00e1nh d\u1ea5u b\u1eb1ng hai thu\u1ed1c nhu\u1ed9m hu\u1ef3nh quang FAM v\u00e0 TAMRA. FAM l\u00e0 thu\u1ed1c nhu\u1ed9m ph\u00e1t hu\u1ef3nh quang v\u00e0 TAMRA l\u00e0 thu\u1ed1c nhu\u1ed9m d\u1eadp t\u1eaft hu\u1ef3nh quang.<\/p>\n\n\n\n

Y\u00eau c\u1ea7u chung v\u1ec1 thu\u1ed1c th\u1eed \u0111\u1ed1i v\u1edbi ch\u1ea5t l\u01b0\u1ee3ng c\u1ee7a thu\u1ed1c th\u1eed \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng trong TCVN 7608 (ISO 24276) – Th\u1ef1c ph\u1ea9m – Ph\u01b0\u01a1ng ph\u00e1p ph\u00e2n t\u00edch \u0111\u1ec3 ph\u00e1t hi\u1ec7n sinh v\u1eadt bi\u1ebfn \u0111\u1ed5i gen v\u00e0 s\u1ea3n ph\u1ea9m c\u00f3 ngu\u1ed3n g\u1ed1c bi\u1ebfn \u0111\u1ed5i gen – Y\u00eau c\u1ea7u chung v\u00e0 \u0111\u1ecbnh ngh\u0129a.<\/p>\n\n\n\n

Thu\u1ed1c th\u1eed ph\u00e2n t\u00edch ph\u1ea3i l\u00e0 lo\u1ea1i tinh khi\u1ebft th\u00edch h\u1ee3p d\u00f9ng cho sinh h\u1ecdc ph\u00e2n t\u1eed, kh\u00f4ng ch\u1ee9a ADN v\u00e0 DNAse. S\u1eed d\u1ee5ng thu\u1ed1c th\u1eed ph\u1ea3i tu\u00e2n theo h\u01b0\u1edbng d\u1eabn c\u1ee7a nh\u00e0 s\u1ea3n xu\u1ea5t. N\u01b0\u1edbc, n\u01b0\u1edbc c\u1ea5t hai l\u1ea7n, n\u01b0\u1edbc \u0111\u00e3 kh\u1eed ion ho\u1eb7c n\u01b0\u1edbc c\u00f3 ch\u1ea5t l\u01b0\u1ee3ng t\u01b0\u01a1ng \u0111\u01b0\u01a1ng. \u0110\u1ec7m ph\u1ea3n \u1ee9ng, c\u00f3 ch\u1ee9a chu\u1ea9n th\u1ee5 \u0111\u1ed9ng ROX, 2x. Dung d\u1ecbch magie clorua, dung d\u1ecbch deoxyribonucleosit triphosphat, enzym ADN polymerase ch\u1ecbu nhi\u1ec7t<\/p>\n\n\n\n

Khi th\u1ef1c hi\u1ec7n th\u1eed c\u1ea7n s\u1eed d\u1ee5ng c\u00e1c thi\u1ebft b\u1ecb, d\u1ee5ng c\u1ee5 c\u1ee7a ph\u00f2ng th\u1eed nghi\u1ec7m sinh h\u1ecdc ph\u00e2n t\u1eed trong su\u1ed1t quy tr\u00ecnh, tr\u1eeb khi c\u00f3 quy \u0111\u1ecbnh kh\u00e1c. M\u1eabu g\u1eedi \u0111\u1ebfn ph\u00f2ng th\u1eed nghi\u1ec7m ph\u1ea3i l\u00e0 m\u1eabu \u0111\u1ea1i di\u1ec7n, m\u1eabu kh\u00f4ng b\u1ecb h\u01b0 h\u1ecfng ho\u1eb7c bi\u1ebfn \u0111\u1ed5i trong su\u1ed1t qu\u00e1 tr\u00ecnh v\u1eadn chuy\u1ec3n ho\u1eb7c b\u1ea3o qu\u1ea3n. <\/p>\n\n\n\n

Khi ph\u00e1t hi\u1ec7n s\u1ef1 ki\u1ec7n ng\u00f4 chuy\u1ec3n gen MIR 604 c\u00e1c \u0111\u01b0\u1eddng khu\u1ebfch \u0111\u1ea1i real-time PCR c\u1ea7n \u0111\u01b0\u1ee3c ki\u1ec3m tra tr\u1ef1c quan v\u1ec1 h\u00ecnh d\u1ea1ng ch\u1eef S \u0111\u1ec3 lo\u1ea1i tr\u1eeb c\u00e1c k\u1ebft qu\u1ea3 d\u01b0\u01a1ng t\u00ednh gi\u1ea3. C\u1ea7n s\u1eed d\u1ee5ng ch\u01b0\u01a1ng tr\u00ecnh ph\u00e2n t\u00edch d\u1eef li\u1ec7u c\u1ee5 th\u1ec3 \u0111\u1ed1i v\u1edbi m\u00e1y chu tr\u00ecnh nhi\u1ec7t real-time t\u01b0\u01a1ng \u1ee9ng \u0111\u1ec3 nh\u1eadn bi\u1ebft c\u00e1c s\u1ea3n ph\u1ea9m PCR. C\u00e1c k\u1ebft qu\u1ea3 khu\u1ebfch \u0111\u1ea1i c\u00f3 th\u1ec3 \u0111\u01b0\u1ee3c bi\u1ec3u th\u1ecb kh\u00e1c nhau, t\u00f9y thu\u1ed9c v\u00e0o thi\u1ebft b\u1ecb \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng.<\/p>\n\n\n\n

Tr\u00ecnh t\u1ef1 \u0111\u00edch s\u1ef1 ki\u1ec7n ng\u00f4 chuy\u1ec3n gen MIR 604 \u0111\u01b0\u1ee3c coi l\u00e0 ph\u00e1t hi\u1ec7n \u0111\u01b0\u1ee3c, n\u1ebfu khi s\u1eed d\u1ee5ng real-time PCR \u0111\u1eb7c hi\u1ec7u s\u1ef1 ki\u1ec7n ng\u00f4 chuy\u1ec3n gen MIR 604, c\u00f3 th\u1ec3 quan s\u00e1t th\u1ea5y \u0111\u01b0\u1eddng cong khu\u1ebfch \u0111\u1ea1i h\u00ecnh ch\u1eef S v\u00e0 gi\u00e1 tr\u1ecb hu\u1ef3nh quang x\u00e1c \u0111\u1ecbnh tr\u01b0\u1edbc \u0111\u00e3 b\u1ecb v\u01b0\u1ee3t qu\u00e1 ng\u01b0\u1ee1ng.<\/p>\n\n\n\n

Vi\u1ec7c thi\u1ebft l\u1eadp c\u00e1c ki\u1ec3m so\u00e1t PCR kh\u00f4ng b\u1ed5 sung ADN (ki\u1ec3m so\u00e1t thu\u1ed1c th\u1eed PCR, ki\u1ec3m so\u00e1t chi\u1ebft \u00e2m t\u00ednh), kh\u00f4ng quan s\u00e1t th\u1ea5y \u0111\u01b0\u1eddng cong khu\u1ebfch \u0111\u1ea1i h\u00ecnh ch\u1eef S v\u00e0 gi\u00e1 tr\u1ecb hu\u1ef3nh quang x\u00e1c \u0111\u1ecbnh tr\u01b0\u1edbc kh\u00f4ng b\u1ecb v\u01b0\u1ee3t qu\u00e1 ng\u01b0\u1ee1ng. Vi\u1ec7c thi\u1ebft l\u1eadp c\u00e1c ki\u1ec3m so\u00e1t khu\u1ebfch \u0111\u1ea1i (ki\u1ec3m so\u00e1t ADN \u0111\u00edch d\u01b0\u01a1ng t\u00ednh, ki\u1ec3m so\u00e1t \u1ee9c ch\u1ebf PCR) th\u00ec thu \u0111\u01b0\u1ee3c c\u00e1c gi\u00e1 tr\u1ecb Ct d\u1ef1 ki\u1ebfn (Ct l\u00e0 \u0111i\u1ec3m c\u1ee7a \u0111\u01b0\u1eddng khu\u1ebfch \u0111\u1ea1i m\u00e0 t\u1ea1i \u0111\u00f3 t\u00edn hi\u1ec7u hu\u1ef3nh quang t\u0103ng l\u00ean tr\u00ean \u0111\u01b0\u1eddng n\u1ec1n ho\u1eb7c v\u01b0\u1ee3t qua ng\u01b0\u1ee1ng c\u00e0i \u0111\u1eb7t \u0111\u00e3 x\u00e1c \u0111\u1ecbnh tr\u01b0\u1edbc).<\/p>\n\n\n\n

Tr\u00ecnh t\u1ef1 \u0111\u00edch s\u1ef1 ki\u1ec7n ng\u00f4 chuy\u1ec3n gen MIR 604 \u0111\u01b0\u1ee3c coi l\u00e0 kh\u00f4ng ph\u00e1t hi\u1ec7n, khi k\u1ebft qu\u1ea3 \u0111\u01b0\u1ee3c bi\u1ec3u th\u1ecb l\u00e0 \u201ckh\u00f4ng x\u00e1c \u0111\u1ecbnh\u201d, \u201ckh\u00f4ng khu\u1ebfch \u0111\u1ea1i\u201d, kh\u00f4ng quan s\u00e1t th\u1ea5y \u0111\u01b0\u1eddng cong khu\u1ebfch \u0111\u1ea1i h\u00ecnh ch\u1eef S cho th\u1ea5y kh\u00f4ng c\u00f3 s\u1ea3n ph\u1ea9m PCR c\u00f3 th\u1ec3 ph\u00e1t hi\u1ec7n \u0111\u01b0\u1ee3c. Ho\u1eb7c gi\u00e1 tr\u1ecb hu\u1ef3nh quang x\u00e1c \u0111\u1ecbnh tr\u01b0\u1edbc chu k\u1ef3 ng\u01b0\u1ee1ng Ct kh\u00f4ng b\u1ecb v\u01b0\u1ee3t qu\u00e1 ng\u01b0\u1ee1ng v\u1edbi chu k\u1ef3 ng\u01b0\u1ee1ng Ct l\u00e0 gi\u00e1 tr\u1ecb hu\u1ef3nh quang Ct m\u1eabu chu\u1ea9n.<\/p>\n\n\n\n

Trong vi\u1ec7c b\u00e1o c\u00e1o th\u1eed nghi\u1ec7m ph\u1ea3i bao g\u1ed3m \u00edt nh\u1ea5t c\u00e1c th\u00f4ng tin c\u1ea7n thi\u1ebft \u0111\u1ec3 nh\u1eadn bi\u1ebft \u0111\u1ea7y \u0111\u1ee7 m\u1eabu th\u1eed; ph\u01b0\u01a1ng ph\u00e1p l\u1ea5y m\u1eabu \u0111\u00e3 s\u1eed d\u1ee5ng, n\u1ebfu bi\u1ebft; ph\u01b0\u01a1ng ph\u00e1p th\u1eed \u0111\u00e3 s\u1eed d\u1ee5ng v\u00e0 vi\u1ec7n d\u1eabn ti\u00eau chu\u1ea9n n\u00e0y; m\u1ecdi thao t\u00e1c kh\u00f4ng \u0111\u01b0\u1ee3c quy \u0111\u1ecbnh trong ti\u00eau chu\u1ea9n n\u00e0y, ho\u1eb7c nh\u1eefng \u0111i\u1ec1u \u0111\u01b0\u1ee3c coi l\u00e0 t\u1ef1 ch\u1ecdn, v\u00e0 b\u1ea5t k\u1ef3 chi ti\u1ebft n\u00e0o c\u00f3 \u1ea3nh h\u01b0\u1edfng t\u1edbi k\u1ebft qu\u1ea3;<\/p>\n\n\n\n

K\u1ebft qu\u1ea3 th\u1eed nghi\u1ec7m thu \u0111\u01b0\u1ee3c, t\u00f9y t\u1eebng tr\u01b0\u1eddng h\u1ee3p c\u1ee5 th\u1ec3 k\u1ebft qu\u1ea3 ph\u1ea3i ghi k\u1ebft qu\u1ea3 m\u1eabu \u00e2m t\u00ednh \u0111\u1ed1i v\u1edbi m\u1eabu A, kh\u00f4ng ph\u00e1t hi\u1ec7n \u0111\u01b0\u1ee3c s\u1ef1 ki\u1ec7n ng\u00f4 chuy\u1ec3n gen MIR 604; K\u1ebft qu\u1ea3 m\u1eabu d\u01b0\u01a1ng t\u00ednh \u0111\u1ed1i v\u1edbi m\u1eabu A, ph\u00e1t hi\u1ec7n \u0111\u01b0\u1ee3c s\u1ef1 ki\u1ec7n ng\u00f4 chuy\u1ec3n gen MIR 604; K\u1ebft qu\u1ea3 \u0111\u1ecbnh l\u01b0\u1ee3ng \u0111\u1ed1i v\u1edbi m\u1eabu A, h\u00e0m l\u01b0\u1ee3ng ADN c\u00f3 ngu\u1ed3n g\u1ed1c t\u1eeb s\u1ef1 ki\u1ec7n ng\u00f4 chuy\u1ec3n gen MIR 604 l\u00e0 X \u00b1 umeas %\u201d, trong \u0111\u00f3 umeas l\u00e0 \u0111\u1ed9 kh\u00f4ng \u0111\u1ea3m b\u1ea3o \u0111o.<\/p>\n\n\n\n

 An D\u01b0\u01a1ng<\/strong><\/p>\n\n\n\n

https:\/\/vietq.vn\/tcvn-13917-22023-phat-hien-va-dinh-luong-ngo-chuyen-gen-mir-604-s26-d225711.html<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"

Ng\u00f4 bi\u1ebfn \u0111\u1ed5i gen hi\u1ec7n v\u1eabn c\u00f2n l\u00e0 \u0111\u1ec1 t\u00e0i tranh c\u00e3i n\u00ean vi\u1ec7c ph\u00e1t hi\u1ec7n v\u00e0 \u0111\u1ecbnh l\u01b0\u1ee3ng th\u1ef1c v\u1eadt s\u1ef1 ki\u1ec7n ng\u00f4 chuy\u1ec3n gen MIR 604 theo Ti\u00eau chu\u1ea9n qu\u1ed1c gia TCVN 13917-2:2023 l\u00e0 \u0111i\u1ec1u c\u1ea7n thi\u1ebft. Ng\u00f4 bi\u1ebfn \u0111\u1ed5i gen l\u00e0 m\u1ed9t s\u1ea3n ph\u1ea9m n\u00f4ng nghi\u1ec7p m\u1edbi \u0111\u01b0\u1ee3c lai t\u1ea1o. C\u00e1ch th\u1ee9c […]<\/p>\n","protected":false},"author":26,"featured_media":36262,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[47],"tags":[],"class_list":["post-36261","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-tin-tuc"],"_links":{"self":[{"href":"https:\/\/casta.ttud.com.vn\/index.php\/wp-json\/wp\/v2\/posts\/36261","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/casta.ttud.com.vn\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/casta.ttud.com.vn\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/casta.ttud.com.vn\/index.php\/wp-json\/wp\/v2\/users\/26"}],"replies":[{"embeddable":true,"href":"https:\/\/casta.ttud.com.vn\/index.php\/wp-json\/wp\/v2\/comments?post=36261"}],"version-history":[{"count":1,"href":"https:\/\/casta.ttud.com.vn\/index.php\/wp-json\/wp\/v2\/posts\/36261\/revisions"}],"predecessor-version":[{"id":36263,"href":"https:\/\/casta.ttud.com.vn\/index.php\/wp-json\/wp\/v2\/posts\/36261\/revisions\/36263"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/casta.ttud.com.vn\/index.php\/wp-json\/wp\/v2\/media\/36262"}],"wp:attachment":[{"href":"https:\/\/casta.ttud.com.vn\/index.php\/wp-json\/wp\/v2\/media?parent=36261"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/casta.ttud.com.vn\/index.php\/wp-json\/wp\/v2\/categories?post=36261"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/casta.ttud.com.vn\/index.php\/wp-json\/wp\/v2\/tags?post=36261"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}