{"id":35607,"date":"2024-08-27T08:49:18","date_gmt":"2024-08-27T01:49:18","guid":{"rendered":"https:\/\/ttud.com.vn\/?p=35607"},"modified":"2024-08-27T08:49:30","modified_gmt":"2024-08-27T01:49:30","slug":"tcvn-8710-82023-ve-quy-trinh-chan-doan-benh-hoai-tu-co-o-tom","status":"publish","type":"post","link":"https:\/\/casta.ttud.com.vn\/index.php\/tcvn-8710-82023-ve-quy-trinh-chan-doan-benh-hoai-tu-co-o-tom\/","title":{"rendered":"TCVN 8710-8:2023 v\u1ec1 quy tr\u00ecnh ch\u1ea9n \u0111o\u00e1n b\u1ec7nh ho\u1ea1i t\u1eed c\u01a1 \u1edf t\u00f4m"},"content":{"rendered":"\n

B\u1ec7nh ho\u1ea1i t\u1eed c\u01a1 \u1edf t\u00f4m l\u00e0 m\u1ed9t b\u1ec7nh truy\u1ec1n nhi\u1ec5m g\u00e2y thi\u1ec7t h\u1ea1i l\u1edbn n\u1ebfu kh\u00f4ng \u0111\u01b0\u1ee3c ch\u1ea9n \u0111o\u00e1n v\u00e0 x\u1eed l\u00fd k\u1ecbp th\u1eddi. Do \u0111\u00f3, vi\u1ec7c ch\u1ea9n \u0111o\u00e1n s\u1edbm c\u1ee7a b\u1ec7nh theo Ti\u00eau chu\u1ea9n qu\u1ed1c gia TCVN 8710-8:2023 s\u1ebd gi\u00fap h\u1ea1n ch\u1ebf \u0111\u01b0\u1ee3c nguy c\u01a1 m\u1ea7m b\u1ec7nh l\u00e2y lan r\u1ed9ng.<\/p>\n\n\n\n

Vi r\u00fat g\u00e2y b\u1ec7nh ho\u1ea1i t\u1eed c\u01a1 IMNV l\u00e0 vi r\u00fat thu\u1ed9c Chi Giardiavirus lamblia, h\u1ecd Totiviridae. IMNV c\u00f3 h\u00ecnh d\u1ea1ng t\u1ee9 di\u1ec7n v\u00e0 \u0111\u01b0\u1eddng k\u00ednh 40nm, m\u1eadt \u0111\u1ed9 n\u1ed5i 1.366 g\/ml. B\u1ed9 gen bao g\u1ed3m m\u1ed9t ph\u00e2n t\u1eed ARN \u0111\u01a1n, chu\u1ed7i k\u00e9p (ds) c\u00f3 k\u00edch th\u01b0\u1edbc 8226-8230 bp.<\/p>\n\n\n\n

C\u00e1c lo\u00e0i t\u00f4m d\u1ec5 nhi\u1ec5m vi r\u00fat nh\u01b0 t\u00f4m th\u1ebb ch\u00e2n tr\u1eafng, t\u00f4m s\u00fa, t\u00f4m s\u00fa n\u00e2u, t\u00f4m chu\u1ed1i, t\u00f4m xanh Nam M\u1ef9 v\u00e0 t\u00f4m n\u00e2u ph\u01b0\u01a1ng nam. C\u00e1c c\u01a1 quan v\u00e0 m\u00f4 \u0111\u00edch b\u1ecb nhi\u1ec5m vi r\u00fat nh\u01b0 c\u01a1 th\u1ecbt (c\u01a1 v\u00e2n), m\u00f4 li\u00ean k\u1ebft, t\u1ebf b\u00e0o m\u00e1u v\u00e0 c\u00e1c t\u1ebf b\u00e0o nhu m\u00f4 c\u1ee7a c\u01a1 quan lymphoid.<\/p>\n\n\n\n

Vi r\u00fat IMNV th\u01b0\u1eddng g\u00e2y t\u1ef7 l\u1ec7 ch\u1ebft cao nh\u1ea5t cho t\u00f4m th\u1ebb ch\u00e2n tr\u1eafng t\u1eeb 40 \u0111\u1ebfn 70% t\u1ed5ng \u0111\u00e0n. IMNV c\u00f3 th\u1ec3 x\u1ea3y ra \u1edf b\u1ea5t k\u1ef3 giai \u0111o\u1ea1n ph\u00e1t tri\u1ec3n n\u00e0o c\u1ee7a t\u00f4m v\u00e0 l\u00e0 m\u1ed9t b\u1ec7nh nhi\u1ec5m tr\u00f9ng m\u00e3n t\u00ednh. T\u1ef7 l\u1ec7 t\u1eed vong c\u00f3 th\u1ec3 l\u00ean t\u1edbi 70% v\u00e0o cu\u1ed1i chu k\u1ef3 nu\u00f4i.<\/p>\n\n\n\n

L\u1ee9a tu\u1ed5i m\u1eafc b\u1ec7nh t\u00f4m th\u1ebb ch\u00e2n tr\u1eafng \u1edf giai \u0111o\u1ea1n t\u00f4m gi\u1ed1ng v\u00e0 ti\u1ec1n tr\u01b0\u1edfng th\u00e0nh th\u01b0\u1eddng nh\u1ea1y c\u1ea3m nh\u1ea5t v\u1edbi m\u1ea7m b\u1ec7nh. B\u1ec7nh c\u00f3 th\u1ec3 l\u00e2y truy\u1ec1n theo chi\u1ec1u ngang, con t\u00f4m b\u1ec7nh l\u00e2y sang con t\u00f4m kh\u1ecfe ho\u1eb7c l\u00e2y truy\u1ec1n qua m\u00f4i tr\u01b0\u1eddng n\u01b0\u1edbc. Nhi\u1ec7t \u0111\u1ed9 v\u00e0 n\u1ed3ng \u0111\u1ed9 mu\u1ed1i \u0111\u01b0\u1ee3c xem l\u00e0 m\u1ed9t trong nh\u1eefng y\u1ebfu t\u1ed1 m\u00f4i tr\u01b0\u1eddng c\u00f3 \u1ea3nh h\u01b0\u1edfng \u0111\u1ebfn qu\u00e1 tr\u00ecnh ph\u00e1t b\u1ec7nh c\u1ee7a b\u1ec7nh ho\u1ea1i t\u1eed c\u01a1.<\/p>\n\n\n\n

T\u00f4m b\u1ecb b\u1ec7nh ho\u1ea1i t\u1eed c\u01a1 \u1edf giai \u0111o\u1ea1n c\u1ea5p t\u00ednh xu\u1ea5t hi\u1ec7n c\u00e1c v\u00f9ng ho\u1ea1i t\u1eed tr\u1eafng r\u1ed9ng \u1edf c\u00e1c c\u01a1 v\u00e2n nh\u01b0 ph\u1ea7n c\u01a1 b\u1ee5ng v\u00e0 c\u01a1 \u0111u\u00f4i, \u0111\u1eb7c bi\u1ec7t l\u00e0 ph\u1ea7n b\u1ee5ng d\u1eabn \u0111\u1ebfn hi\u1ec7n t\u01b0\u1ee3ng ho\u1ea1i t\u1eed. Sau khi ch\u1ebft c\u00e1c ph\u1ea7n n\u00e0y c\u00f3 m\u00e0u \u0111\u1ecf t\u01b0\u01a1ng t\u1ef1 nh\u01b0 m\u00e0u c\u1ee7a t\u00f4m n\u1ea5u ch\u00edn.<\/p>\n\n\n\n

T\u00f4m nhi\u1ec5m IMNV tr\u1edf n\u00ean l\u1edd \u0111\u1edd v\u00e0 gi\u1ea3m \u0103n, c\u00f3 m\u00e0u tr\u1eafng \u0111\u1ee5c c\u01a1, th\u01b0\u1eddng n\u1ed5i b\u1eadt h\u01a1n \u1edf t\u00f4m th\u1ebb ch\u00e2n tr\u1eafng so v\u1edbi t\u00f4m xanh v\u00e0 t\u00f4m s\u00fa. K\u00edch th\u01b0\u1edbc c\u1ee7a c\u01a1 quan b\u1ea1ch huy\u1ebft c\u1ee7a t\u00f4m nhi\u1ec5m IMNV t\u0103ng g\u1ea5p 2 \u0111\u1ebfn 4 l\u1ea7n so v\u1edbi k\u00edch th\u01b0\u1edbc th\u00f4ng th\u01b0\u1eddng.<\/p>\n\n\n\n

Tuy nhi\u00ean vi\u1ec7c x\u00e1c \u0111\u1ecbnh b\u1ec7nh ho\u1ea1i t\u1eed c\u01a1 \u1edf t\u00f4m s\u1edbm trong nu\u00f4i tr\u1ed3ng th\u1ee7y s\u1ea3n kh\u00f4ng ph\u1ea3i \u0111\u01a1n gi\u1ea3n. Do \u0111\u00f3 \u0111\u1ec3 x\u00e1c \u0111\u1ecbnh \u0111\u01b0\u1ee3c b\u1ec7nh k\u1ecbp th\u1eddi v\u00e0 ch\u00ednh x\u00e1c n\u00ean tu\u00e2n th\u1ee7 theo h\u01b0\u1edbng d\u1eabn t\u1ea1i Ti\u00eau chu\u1ea9n qu\u1ed1c gia TCVN 8710-8:2023 B\u1ec7nh th\u1ee7y s\u1ea3n – Quy tr\u00ecnh ch\u1ea9n \u0111o\u00e1n – Ph\u1ea7n 8 b\u1ec7nh ho\u1ea1i t\u1eed c\u01a1 \u1edf t\u00f4m do B\u1ed9 Khoa h\u1ecdc v\u00e0 C\u00f4ng ngh\u1ec7 c\u00f4ng b\u1ed1. <\/p>\n\n\n\n

\"\"<\/figure><\/div>\n\n\n\n

Vi\u1ec7c x\u00e1c \u0111\u1ecbnh b\u1ec7nh ho\u1ea1i t\u1eed \u1edf t\u00f4m theo h\u01b0\u1edbng d\u1eabn c\u1ee7a ti\u00eau chu\u1ea9n qu\u1ed1c gia gi\u00fap h\u1ea1n ch\u1ebf \u0111\u01b0\u1ee3c m\u1ea7m b\u1ec7nh l\u00e2y truy\u1ec1n. \u1ea2nh minh h\u1ecda<\/em><\/h2>\n\n\n\n

V\u1ec1 ch\u1ea9n \u0111o\u00e1n l\u00e2m s\u00e0ng, tr\u01b0\u1edbc h\u1ebft ti\u00eau chu\u1ea9n n\u00e0y ch\u1ec9 ra c\u00e1c \u0111\u1eb7c \u0111i\u1ec3m d\u1ecbch t\u1ec5 \u0111\u1ec3 nh\u1eadn bi\u1ebft b\u1ec7nh ho\u1ea1i t\u1eed c\u01a1 \u1edf t\u00f4m. \u0110\u00e2y l\u00e0 b\u1ec7nh truy\u1ec1n nhi\u1ec5m do t\u00e1c nh\u00e2n infectious myonecrosis virus (IMNV) g\u00e2y ra \u1edf t\u00f4m. T\u00f4m b\u1ecb b\u1ec7nh ho\u1ea1i t\u1eed c\u01a1 ban \u0111\u1ea7u c\u01a1 \u0111u\u00f4i c\u00f3 hi\u1ec7n t\u01b0\u1ee3ng m\u00e0u tr\u1eafng \u0111\u1ee5c s\u1eefa, sau \u0111\u00f3 lan d\u1ea7n kh\u1eafp c\u01a1 th\u1ec3. T\u00f4m b\u1ecb b\u1ec7nh n\u1eb7ng c\u00f3 hi\u1ec7n t\u01b0\u1ee3ng ho\u1ea1i t\u1eed v\u00e0 \u0111\u1ecf \u1edf ph\u1ea7n c\u01a1 n\u00e0y.<\/p>\n\n\n\n

Quy tr\u00ecnh ch\u1ea9n \u0111o\u00e1n b\u1ec7nh b\u1eb1ng c\u00e1ch l\u1ea5y m\u1eabu \u0111\u1ec3 x\u00e1c \u0111\u1ecbnh, theo \u0111\u00f3 s\u1ed1 l\u01b0\u1ee3ng t\u00f4m tr\u00ean m\u1ed7i m\u1eabu ph\u1ee5 thu\u1ed9c v\u00e0o k\u00edch c\u1ee1 c\u1ee7a t\u00f4m. \u0110\u1ed1i v\u1edbi t\u00f4m gi\u1ed1ng, \u1ea5u tr\u00f9ng v\u00e0 h\u1eadu \u1ea5u tr\u00f9ng n\u00ean l\u1ea5y nguy\u00ean con, t\u1eeb 100 mg \u0111\u1ebfn 500mg\/ m\u1eabu. T\u00f4m th\u01b0\u01a1ng ph\u1ea9m l\u1ea5y nguy\u00ean con, t\u1eeb 5 con\/m\u1eabu \u0111\u1ebfn 10 con\/m\u1eabu ho\u1eb7c l\u1ea5y mang, m\u00e1u, bi\u1ec3u m\u00f4 d\u01b0\u1edbi v\u1ecf kitin, ch\u00e2n b\u01a1i c\u1ee7a 5 con\/m\u1eabu \u0111\u1ebfn 10 con\/m\u1eabu. T\u00f4m b\u1ed1 m\u1eb9 n\u00ean l\u1ea5y nguy\u00ean con, 1 con\/m\u1eabu ho\u1eb7c l\u1ea5y mang, ch\u00e2n b\u01a1i c\u1ee7a 1 con\/m\u1eabu.<\/p>\n\n\n\n

Thu m\u1eabu t\u00f4m c\u00f3 d\u1ea5u hi\u1ec7u b\u1ec7nh l\u00fd, c\u00f2n s\u1ed1ng ho\u1eb7c m\u1edbi ch\u1ebft. M\u1eabu b\u1ec7nh ph\u1ea9m ph\u1ea3i \u0111\u01b0\u1ee3c l\u1ea5y v\u00f4 tr\u00f9ng v\u00e0 \u0111\u1ec3 ri\u00eang bi\u1ec7t trong d\u1ee5ng c\u1ee5 ch\u1ee9a m\u1eabu. B\u1ea3o qu\u1ea3n m\u1eabu \u1edf nhi\u1ec7t \u0111\u1ed9 t\u1eeb 2\u00b0C \u0111\u1ebfn 8 \u00b0C kh\u00f4ng qu\u00e1 48 h t\u1eeb khi l\u1ea5y m\u1eabu \u0111\u1ebfn khi v\u1eadn chuy\u1ec3n v\u1ec1 ph\u00f2ng th\u00ed nghi\u1ec7m ho\u1eb7c b\u1ea3o qu\u1ea3n trong ethanol 70 % \u0111\u1ebfn ethanol tuy\u1ec7t \u0111\u1ed1i v\u1edbi t\u1ef7 l\u1ec7 1:10.<\/p>\n\n\n\n

M\u1eabu \u0111\u01b0\u1ee3c b\u1ea3o qu\u1ea3n \u1edf nhi\u1ec7t \u0111\u1ed9 t\u1eeb 2 \u00b0C \u0111\u1ebfn 8 \u00b0C chuy\u1ec3n \u0111\u1ebfn ph\u00f2ng th\u00ed nghi\u1ec7m ch\u01b0a ph\u00e2n t\u00edch ngay ph\u1ea3i \u0111\u01b0\u1ee3c b\u1ea3o qu\u1ea3n \u1edf nhi\u1ec7t \u0111\u1ed9 \u00e2m 20 \u00b0C \u0111\u1ebfn \u00e2m 80 \u00b0C ho\u1eb7c \u0111\u01b0\u1ee3c b\u1ea3o qu\u1ea3n trong dung d\u1ecbch PBS (dung d\u1ecbch mu\u1ed1i c\u00e2n b\u1eb1ng) theo t\u1ef7 l\u1ec7 1:10 (1 ph\u1ea7n m\u1eabu: 9 ph\u1ea7n PBS) \u1edf nhi\u1ec7t \u0111\u1ed9 \u00e2m 20 \u00b0C \u0111\u1ebfn \u00e2m 80 \u00b0C ho\u1eb7c b\u1ea3o qu\u1ea3n trong ethanol t\u1eeb 70 % \u0111\u1ebfn ethanol tuy\u1ec7t \u0111\u1ed1i.<\/p>\n\n\n\n

\u0110\u1ec3 chu\u1ea9n b\u1ecb m\u1eabu n\u00ean d\u00f9ng panh, k\u00e9o v\u00f4 tr\u00f9ng \u0111\u1ec3 th\u1ef1c hi\u1ec7n c\u00e1c thao t\u00e1c t\u00e1ch, c\u1eaft l\u1ea5y m\u1eabu. M\u1eabu \u0111\u01b0\u1ee3c chia th\u00e0nh hai ph\u1ea7n, m\u1ed9t ph\u1ea7n cho th\u1ef1c hi\u1ec7n x\u00e9t nghi\u1ec7m v\u00e0 m\u1ed9t ph\u1ea7n l\u01b0u tr\u1eef. L\u01b0\u1ee3ng m\u1eabu l\u1ea5y \u0111\u1ec3 t\u00e1ch chi\u1ebft ARN kho\u1ea3ng 30 mg. Cho 30 mg m\u1eabu v\u00e0o dung d\u1ecbch PBS v\u1edbi t\u1ef7 l\u1ec7 1:10 \u0111\u1ed3ng nh\u1ea5t m\u1eabu b\u1eb1ng m\u00e1y nghi\u1ec1n m\u1eabu ho\u1eb7c b\u1eb1ng c\u1ed1i, ch\u00e0y nghi\u1ec1n m\u1eabu \u0111\u1ec3 t\u1ea1o th\u00e0nh huy\u1ec5n d\u1ecbch 10 %. Chuy\u1ec3n huy\u1ec5n d\u1ecbch v\u00e0o 2 \u1ed1ng v\u00f4 tr\u00f9ng \u0111\u00f3ng n\u1eafp, m\u1ed9t \u1ed1ng m\u1eabu \u0111\u1ec3 x\u00e9t nghi\u1ec7m v\u00e0 m\u1ed9t \u1ed1ng l\u01b0u m\u1eabu. L\u01b0u \u00fd v\u1edbi m\u1eabu \u0111\u00e3 b\u1ea3o qu\u1ea3n \u1edf nhi\u1ec7t \u0111\u1ed9 \u00e2m 20 \u00b0C \u0111\u1ebfn \u00e2m 80 \u00b0C c\u1ea7n r\u00e3 \u0111\u00f4ng ngo\u00e0i nhi\u1ec7t \u0111\u1ed9 ph\u00f2ng tr\u01b0\u1edbc khi th\u1ef1c hi\u1ec7n \u0111\u1ed3ng nh\u1ea5t m\u1eabu. Y\u00eau c\u1ea7u v\u1ec1 t\u00e1ch chi\u1ebft ARN n\u00ean s\u1eed d\u1ee5ng b\u1ed9 k\u00edt t\u00e1ch chi\u1ebft th\u00edch h\u1ee3p v\u00e0 an to\u00e0n theo h\u01b0\u1edbng d\u1eabn c\u1ee7a nh\u00e0 s\u1ea3n xu\u1ea5t.<\/p>\n\n\n\n

S\u1eed d\u1ee5ng ph\u01b0\u01a1ng ph\u00e1p Nested RT-PCR- Nested RTPCR (l\u00e0 ph\u01b0\u01a1ng ph\u00e1p ch\u1ea9n \u0111o\u00e1n nhanh, v\u1edbi \u0111\u1ed9 nh\u1ea1y v\u00e0 \u0111\u1ed9 \u0111\u1eb7c hi\u1ec7u cao, \u0111\u1ecbnh ARN trong nhi\u1ec1u lo\u1ea1i m\u1eabu b\u1ec7nh ph\u1ea9m kh\u00e1c nhau. K\u1ebft qu\u1ea3 c\u1ee7a x\u00e9t nghi\u1ec7m nh\u1eadn \u0111\u01b0\u1ee3c trong v\u00f2ng 24 gi\u1edd k\u1ec3 t\u1eeb khi nh\u1eadn m\u1eabu, ng\u01b0\u1ee3c l\u1ea1i, ph\u01b0\u01a1ng ph\u00e1p ph\u00e2n l\u1eadp vi r\u00fat ph\u1ea3i m\u1ea5t 3 hay 4 tu\u1ea7n) s\u1eed d\u1ee5ng c\u1eb7p m\u1ed3i \u0111\u1eb7c hi\u1ec7u \u0111\u1ec3 ph\u00e1t hi\u1ec7n vi r\u00fat g\u00e2y ho\u1ea1i t\u1eed c\u01a1 \u1edf t\u00f4m.<\/p>\n\n\n\n

M\u1ed3i \u1edf tr\u1ea1ng th\u00e1i \u0111\u00f4ng kh\u00f4 ph\u1ea3i \u0111\u01b0\u1ee3c ly t\u00e2m nhanh b\u1eb1ng m\u00e1y ly t\u00e2m trong 30 s \u0111\u1ec3 m\u1ed3i l\u1eafng xu\u1ed1ng \u0111\u00e1y \u1ed1ng tr\u01b0\u1edbc khi m\u1edf v\u00e0 ho\u00e0n nguy\u00ean. D\u00f9ng dung d\u1ecbch \u0111\u1ec7m TE \u0111\u1ec3 ho\u00e0n nguy\u00ean m\u1ed3i \u1edf n\u1ed3ng \u0111\u1ed9 100 \u03bcm l\u00e0m g\u1ed1c. M\u1ed3i \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng \u1edf n\u1ed3ng \u0111\u1ed9 20 \u03bcm sau \u0111\u00f3 pha lo\u00e3ng m\u1ed3i g\u1ed1c b\u1eb1ng n\u01b0\u1edbc tinh khi\u1ebft<\/p>\n\n\n\n

Ph\u1ea3n \u1ee9ng khu\u1ebfch \u0111\u1ea1i \u0111\u01b0\u1ee3c th\u1ef1c hi\u1ec7n trong m\u00e1y nh\u00e2n gen PCR theo ph\u01b0\u01a1ng ph\u00e1p Nested RT- PCR khu\u1ebfch \u0111\u1ea1i \u0111o\u1ea1n gen \u0111\u1eb7c hi\u1ec7u c\u1ee7a vi r\u00fat g\u00e2y b\u1ec7nh ho\u1ea1i t\u1eed c\u01a1 \u1edf t\u00f4m s\u1eed d\u1ee5ng c\u1eb7p m\u1ed3i 4587F\/ 4914R v\u00e0 4725F\/4863R.<\/p>\n\n\n\n

Ph\u01b0\u01a1ng ph\u00e1p t\u00e1ch chi\u1ebft ARN realtime RT – PCR b\u1eb1ng c\u00e1ch s\u1eed d\u1ee5ng b\u1ed9 k\u00edt t\u00e1ch chi\u1ebft ARN th\u00edch h\u1ee3p v\u00e0 an to\u00e0n theo h\u01b0\u1edbng d\u1eabn c\u1ee7a nh\u00e0 s\u1ea3n xu\u1ea5t. Ph\u1ea3n \u1ee9ng khu\u1ebfch \u0111\u1ea1i \u0111\u01b0\u1ee3c th\u1ef1c hi\u1ec7n trong m\u00e1y realtime PCR theo ph\u01b0\u01a1ng ph\u00e1p realtime RT- PCR khu\u1ebfch \u0111\u1ea1i \u0111o\u1ea1n gen \u0111\u1eb7c hi\u1ec7u c\u1ee7a vi r\u00fat g\u00e2y ho\u1ea1i t\u1eed c\u01a1 s\u1eed d\u1ee5ng c\u1eb7p m\u1ed3i IMNV142F \/ IMNV545R v\u00e0 \u0111o\u1ea1n d\u00f2 Taqman IMNV-p1.<\/p>\n\n\n\n

M\u1ed3i \u1edf tr\u1ea1ng th\u00e1i \u0111\u00f4ng kh\u00f4 ph\u1ea3i \u0111\u01b0\u1ee3c ly t\u00e2m nhanh b\u1eb1ng m\u00e1y ly t\u00e2m trong 30 s \u0111\u1ec3 m\u1ed3i l\u1eafng xu\u1ed1ng \u0111\u00e1y \u1ed1ng tr\u01b0\u1edbc khi m\u1edf v\u00e0 ho\u00e0n nguy\u00ean. Khi ho\u00e0n nguy\u00ean, n\u00ean d\u00f9ng dung d\u1ecbch \u0111\u1ec7m TE \u0111\u1ec3 ho\u00e0n nguy\u00ean m\u1ed3i \u1edf n\u1ed3ng \u0111\u1ed9 100 \u03bcM l\u00e0m g\u1ed1c. M\u1ed3i \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng \u1edf n\u1ed3ng \u0111\u1ed9 20 \u03bcM pha lo\u00e3ng m\u1ed3i g\u1ed1c b\u1eb1ng n\u01b0\u1edbc tinh khi\u1ebft kh\u00f4ng c\u00f3 nuclease (l\u00e0 m\u1ed9t lo\u1ea1i enzym c\u00f3 kh\u1ea3 n\u0103ng c\u1eaft li\u00ean k\u1ebft).<\/p>\n\n\n\n

Ph\u01b0\u01a1ng ph\u00e1p ki\u1ec3m tra b\u1ec7nh t\u00edch vi th\u1ec3 b\u1eb1ng ph\u01b0\u01a1ng ph\u00e1p nhu\u1ed9m HE n\u00ean l\u1ea5y m\u1eabu b\u1eb1ng c\u00e1ch t\u00f4m \u1ea5u tr\u00f9ng, h\u1eadu \u1ea5u tr\u00f9ng s\u1eed d\u1ee5ng nguy\u00ean con. T\u00f4m b\u1ed1 m\u1eb9, t\u00f4m th\u01b0\u01a1ng ph\u1ea9m l\u1ea5y nguy\u00ean con ho\u1eb7c b\u1ed9 ph\u1eadn \u0111\u1ea7u ng\u1ef1c c\u00f3 ch\u1ee9a kh\u1ed1i gan t\u1ee5y (< 1 cm3), c\u1ed1 \u0111\u1ecbnh m\u1eabu trong dung d\u1ecbch th\u1eed Davidson ngay sau khi l\u1ea5y m\u1eabu v\u1edbi t\u1ef7 l\u1ec7 m\u1eabu v\u00e0 dung d\u1ecbch Davidson. M\u1eabu c\u1ed1 \u0111\u1ecbnh trong Davidson chuy\u1ec3n \u0111\u1ebfn ph\u00f2ng th\u00ed nghi\u1ec7m trong kho\u1ea3ng t\u1eeb 24 h \u0111\u1ebfn 72 h sau khi l\u1ea5y m\u1eabu. M\u1eabu c\u1ed1 \u0111\u1ecbnh trong Davidson kho\u1ea3ng t\u1eeb 24 h \u0111\u1ebfn 72 h. M\u1eabu \u0111\u01b0\u1ee3c chuy\u1ec3n sang ethanol 70%. L\u1ea5y b\u1ec7nh ph\u1ea9m \u0111\u00e3 c\u1ed1 \u0111\u1ecbnh trong ethanol ho\u1eb7c Davidson c\u1eaft mi\u1ebfng nh\u1ecf d\u00e0y kho\u1ea3ng 1 mm, d\u00e0i kho\u1ea3ng 1 cm cho v\u00e0o khu\u00f4n nh\u1ef1a.<\/p>\n\n\n\n

Ti\u1ebfn h\u00e0nh x\u1eed l\u00fd m\u1eabu b\u1eb1ng c\u00e1ch c\u1eaft ti\u00eau b\u1ea3n v\u00e0 nhu\u1ed9m ti\u00eau b\u1ea3n ti\u00eau b\u1ea3n Haematoxylin v\u00e0 Eosin theo h\u01b0\u1edbng d\u1eabn. \u0110\u1ecdc k\u1ebft qu\u1ea3 b\u1eb1ng c\u00e1ch soi k\u00ednh hi\u1ec3n vi t\u1eeb v\u1eadt k\u00ednh c\u00f3 \u0111\u1ed9 ph\u00f3ng \u0111\u1ea1i th\u1ea5p \u0111\u1ebfn v\u1eadt k\u00ednh c\u00f3 \u0111\u1ed9 ph\u00f3ng \u0111\u1ea1i cao. Khi t\u00f4m b\u1ecb b\u1ec7nh IMNV, k\u1ebft qu\u1ea3 m\u00f4 b\u1ec7nh h\u1ecdc cho th\u1ea5y c\u00e1c v\u1ecb tr\u00ed ho\u1ea1i t\u1eed \u1edf c\u00e1c s\u1ee3i c\u01a1 v\u00e2n v\u00e0 c\u00f3 hi\u1ec7n t\u01b0\u1ee3ng vi\u00eam, ph\u00f9, xu\u1ea5t huy\u1ebft gi\u1eefa c\u00e1c s\u1ee3i c\u01a1, xu\u1ea5t hi\u1ec7n th\u1ec3 \u1ea9n trong t\u1ebf b\u00e0o ch\u1ea5t. T\u00f4m b\u1ecb nhi\u1ec5m IMNV n\u1eb7ng, ph\u1ea7n c\u01a1 \u1edf b\u1ee5ng b\u1ecb ho\u1ea1i t\u1eed nghi\u00eam tr\u1ecdng v\u00e0 s\u1ef1 x\u00e2m nh\u1eadp v\u1edbi s\u1ed1 l\u01b0\u1ee3ng l\u1edbn c\u1ee7a c\u00e1c t\u1ebf b\u00e0o m\u00e1u v\u00e0o v\u00f9ng b\u1ecb vi\u00eam.<\/p>\n\n\n\n

M\u1eabu t\u00f4m \u0111\u01b0\u1ee3c x\u00e1c \u0111\u1ecbnh m\u1eafc b\u1ec7nh ho\u1ea1i t\u1eed c\u01a1 (IMNV) khi c\u00f3 nh\u1eefng \u0111\u1eb7c \u0111i\u1ec3m d\u1ecbch t\u1ec5, tri\u1ec7u ch\u1ee9ng l\u00e2m s\u00e0ng, b\u1ec7nh t\u00edch \u0111\u1eb7c tr\u01b0ng c\u1ee7a b\u1ec7nh v\u00e0 c\u00f3 k\u1ebft qu\u1ea3 d\u01b0\u01a1ng t\u00ednh v\u1edbi IMNV b\u1eb1ng ph\u01b0\u01a1ng ph\u00e1p Nested RT- PCR ho\u1eb7c c\u00f3 k\u1ebft qu\u1ea3 d\u01b0\u01a1ng t\u00ednh v\u1edbi IMNV b\u1eb1ng ph\u01b0\u01a1ng ph\u00e1p realtime RT- PCR ho\u1eb7c ph\u00e1t hi\u1ec7n \u0111\u01b0\u1ee3c b\u1ec7nh t\u00edch vi th\u1ec3 \u0111\u1eb7c tr\u01b0ng b\u1eb1ng ph\u01b0\u01a1ng ph\u00e1p nhu\u1ed9m HE.<\/p>\n\n\n\n

An D\u01b0\u01a1ng<\/strong><\/p>\n\n\n\n

https:\/\/vietq.vn\/tcvn-8710-82023-ve-quy-trinh-chan-doan-benh-hoai-tu-co-o-tom-d224669.html<\/a><\/p>\n\n\n\n

<\/p>\n","protected":false},"excerpt":{"rendered":"

B\u1ec7nh ho\u1ea1i t\u1eed c\u01a1 \u1edf t\u00f4m l\u00e0 m\u1ed9t b\u1ec7nh truy\u1ec1n nhi\u1ec5m g\u00e2y thi\u1ec7t h\u1ea1i l\u1edbn n\u1ebfu kh\u00f4ng \u0111\u01b0\u1ee3c ch\u1ea9n \u0111o\u00e1n v\u00e0 x\u1eed l\u00fd k\u1ecbp th\u1eddi. Do \u0111\u00f3, vi\u1ec7c ch\u1ea9n \u0111o\u00e1n s\u1edbm c\u1ee7a b\u1ec7nh theo Ti\u00eau chu\u1ea9n qu\u1ed1c gia TCVN 8710-8:2023 s\u1ebd gi\u00fap h\u1ea1n ch\u1ebf \u0111\u01b0\u1ee3c nguy c\u01a1 m\u1ea7m b\u1ec7nh l\u00e2y lan r\u1ed9ng. Vi r\u00fat g\u00e2y […]<\/p>\n","protected":false},"author":26,"featured_media":35608,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[47],"tags":[],"class_list":["post-35607","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-tin-tuc"],"_links":{"self":[{"href":"https:\/\/casta.ttud.com.vn\/index.php\/wp-json\/wp\/v2\/posts\/35607","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/casta.ttud.com.vn\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/casta.ttud.com.vn\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/casta.ttud.com.vn\/index.php\/wp-json\/wp\/v2\/users\/26"}],"replies":[{"embeddable":true,"href":"https:\/\/casta.ttud.com.vn\/index.php\/wp-json\/wp\/v2\/comments?post=35607"}],"version-history":[{"count":1,"href":"https:\/\/casta.ttud.com.vn\/index.php\/wp-json\/wp\/v2\/posts\/35607\/revisions"}],"predecessor-version":[{"id":35609,"href":"https:\/\/casta.ttud.com.vn\/index.php\/wp-json\/wp\/v2\/posts\/35607\/revisions\/35609"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/casta.ttud.com.vn\/index.php\/wp-json\/wp\/v2\/media\/35608"}],"wp:attachment":[{"href":"https:\/\/casta.ttud.com.vn\/index.php\/wp-json\/wp\/v2\/media?parent=35607"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/casta.ttud.com.vn\/index.php\/wp-json\/wp\/v2\/categories?post=35607"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/casta.ttud.com.vn\/index.php\/wp-json\/wp\/v2\/tags?post=35607"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}