{"id":30611,"date":"2020-06-23T22:40:00","date_gmt":"2020-06-23T15:40:00","guid":{"rendered":"https:\/\/ttud.com.vn\/?p=30611"},"modified":"2021-11-28T22:42:23","modified_gmt":"2021-11-28T15:42:23","slug":"overview-of-research-on-dna-barcoding-on-fruit-trees-in-vietnam","status":"publish","type":"post","link":"https:\/\/casta.ttud.com.vn\/index.php\/overview-of-research-on-dna-barcoding-on-fruit-trees-in-vietnam\/","title":{"rendered":"Overview of research on DNA barcoding  on fruit trees in Vietnam"},"content":{"rendered":"\n<p>Regarding DNA analysis of fruit trees, over the years, there have been quite a few studies on genetic diversity of fruit trees in Vietnam, especially in the Southern region, the typical publications are as follows:<\/p>\n\n\n\n<p>Nguyen Loc Hien and ctv. (2012) conducted a study to evaluate genetic diversity through morphology and DNA molecular markers on Mangosteen populations grown in Lai Thieu &#8211; Thuan An area, Binh Duong province. Based on morphology, there are 24\/32 polymorphic traits, accounting for 75%. However, genetic analysis by molecular marker RAPD showed that the degree of polymorphism was not high, expressed in a similarity coefficient of 0.42 &#8211; 1.00. The combined analysis of morphological characteristics and molecular markers showed a high degree of similarity about 0.55 &#8211; 0.99. This result once again confirms the conclusion of previous studies about the high degree of similarity of the Mangosteen population, but also demonstrates the genetic diversity and the ability to provide valuable genetic variation. of Mangosteen populations in Vietnam.<\/p>\n\n\n\n<div class=\"wp-block-image\"><figure class=\"aligncenter\"><img decoding=\"async\" src=\"https:\/\/ttud.com.vn\/wp-content\/uploads\/2021\/11\/Hinh-bai-3-DNA-cay-an-trai-1024x583.jpg\" alt=\"\"\/><\/figure><\/div>\n\n\n\n<p>Tran Nhan Dung and Do Tan Khang (2012) conducted genetic diversity analysis by Amplified Fragments Length Polymorphism (AFLP) and ITS (Internal Transcribed Spacer)-based sequencing techniques on 36 mango cultivars. collected from several provinces of Vietnam, mostly concentrated in Dong Thap province. The analysis results showed that 149 AFLP markers were recorded, of which 49 high frequency markers were found in all mango cultivars with genetic correlation r = 0.853. Combining the correlation analysis AFLP and ITS, the results show that the two varieties of Da mango and Gao mango are only one; two varieties of Bom mango and the Australian Kensington Pride mango are just one. Nha Trang Thuy Trieu mango and similar varieties in Nha Trang have the same origin as Thanh Ca mango in the South. The Bac-Tam-Bang mango, a favorite cultivar of Cambodians, is a form of the Hon Xanh 19 mango. Cat Chu mangoes have many different phenotypes and genotypes. Thanh Ca mango may be the ancestor of many popular mango varieties such as Tuong, Thom, and Cat Chu mangoes. Thai green mango varieties have their own phenotype and genotype different from Vietnamese mango varieties. Particularly, the Manduongcao mango variety contains genes similar to the Tuong mango. Yen Chau mango in Northwest Vietnam has the same origin as the Australian Kensington Pride mango and Bom mango, two identical mango varieties originating from Malaysia and Oceania.<\/p>\n\n\n\n<p>Le Van Hau and ctv. (2018) identified bo bo (Moina sp.) samples in five provinces in the Mekong Delta region (An Giang, Dong Thap, Can Tho, Ben Tre and Long An) by both morphology and The barcoded DNA marker is located on the mitochondrial cytochrome coxidase subunit I (mtCOI) gene. The study has successfully sequenced the mtCOI gene fragment of 48 samples of Moina sp. can be isolated. The analysis results showed that M. micrura species was most present, followed by M. macrocopa species. Samples of Moina spp. classified into five genetic groups; in which, 31\/48 samples belonging to group I had 99% similarity with M. micrura; 4\/48 samples belonging to group V had 99% similarity with species of M. macrocopa; 8\/48 samples in group II, 4\/48 samples in group III and 1\/48 in group IV contained mtCOI gene sequences with 90% lower similarity compared with Moina species published on the Information Center National Biotechnology (NCBI), USA should be likely to be new species that do not yet have an up-to-date mtCOI sequence on the gene bank.<\/p>\n\n\n\n<p>Hoang Minh Trang (2016) identified and analyzed the sequences of a number of barcode segments including rbcL, matK, ycf1b, trnH &#8211; psbA and ITS2 in order to propose suitable barcodes specific for Camellia species. in Tam Dao National Park, namely 5 species of yellow sea sugar (Camellia tienii Ninh), Hakoda yellow tea (Camellia hakoda Ninh, Tr.), thick leaf yellow tea (Camellia crassiphylla Ninh et Hakoda), Tam Dao yellow tea (Camellia crassiphylla Ninh et Hakoda) Camellia tamdaoesis Hakoda et Ninh) and Petelo Yellow Tea (Camellia petelotii (Merr.) Sealy).<\/p>\n\n\n\n<p>Tran Nhan Dung and Tran Thi Le Quyen (2012) conducted genetic diversity testing of 32 mangosteen lines in Binh Duong by ISSR technique with 11 primer pairs. PCR results have amplified 87 bands, of which 40 bands show polymorphism (45.98%) and 47 monomorphic bands (54.02%). Out of 11 primers used, 10 primers gave polymorphic results, especially ISSRED-14 primer gave high polymorphic results, which can be useful primers to study genetic differences between mangosteen lines on the same site. geographical location. Analysis results using NTSYSpc 2.11a software by UPGMA method showed that the similarity level of 32 mangosteen lines based on ISSR molecular markers ranged from 0.75 to 1.00. The study showed that there are genetic differences among 32 lines of mangosteen in Binh Duong although mangosteen has asexual reproduction. This genetic variation may be due to the accumulation of natural mutations to adapt to the environment.<\/p>\n\n\n\n<p>Lieu Nhu Y and Tran Nhan Dung (2012) conducted a genetic diversity survey of 16 edible mushroom strains in the Mekong Delta provinces. Two pairs of primers ITS1 and ITS4 were used to amplify the ITS1 + 5.8S + ITS2 region. The results determined that the samples of the studied straw mushrooms belonged to Volvariella volvaceae species, the white abalone mushroom samples belonged to Pleurotus ostreatus species, the Japanese abalone mushroom samples belonged to the species Pleurotus cytidiosus, the gray abalone mushrooms belonged to the species Pleurotus pulmonarius, two samples Enthusiasm belongs to the species Flammulina velutipes. Reishi mushroom samples belong to 3 species: Ganoderma lucidum, G. gibbosum and G. tropicum. The pedigree chart also shows the genetic correlation between the surveyed fungal species with high bootstrap index, so it can be concluded that these species have a very close origin.<\/p>\n\n\n\n<p>Orientation for development of fruit tree production in the Southern region to 2020 is to form a key fruit-growing area for concentrated planting and to orient the cropping of some fruit trees in the direction of ensuring productivity, quality, and hygiene. food safety, improving efficiency and competitiveness, on the basis of innovating methods of approaching the market, combining fast application of scientific and technological achievements, effectively exploiting advantages and controlling food safety. ecological conditions of each region, each locality for each type of tree; interested in famous specialty fruit trees with high competitive advantages in each locality.<\/p>\n\n\n\n<p>To meet the needs of integration and maintain market share in the international market, Vietnam&#8217;s fruits need to be guaranteed in terms of safety, quality and appearance, in which traceability is a very important issue. important. Furthermore, proving the quality of fruit produced from the original variety or the progeny is extremely complex and difficult, so a reliable method is needed to solve this problem. Therefore, building a barcoded DNA database for Vietnam&#8217;s specialty fruit trees is an extremely important and urgent task today.<\/p>\n\n\n\n<p>References<\/p>\n\n\n\n<p>Hoang Minh Trang (2016) Identification of some DNA barcodes for some yellow tea species in Tam Dao National Park. Thesis Master of Science. Ha Noi national university.<\/p>\n\n\n\n<p>Khuat Thi Mai Luong, Nguyen Thi Minh Nguyet, Chu Duc Ha, Tran Thi Hoa My, Dinh Van Phe and Le Hung Linh. (2018) Design and build a set of molecular markers for genetic testing and research of Ngoc Linh ginseng (Panax vietnamensis). The project belongs to the Ministry of Science and Technology (subject code: \u0110L.CN-29\/16).<\/p>\n\n\n\n<p>Le Van Hau, Le Luu Phuong Hanh, Ngo Huynh Phuong Thao, Nguyen Phuc Cam Tu and Nguyen Quoc Binh. (2018) Application of molecular marker DNA barcode in identification of samples Moina spp. isolated in the Mekong Delta. Scientific Journal of Can Tho University. 54(2): 36-44.<\/p>\n\n\n\n<p>Lieu Nhu Y and Tran Nhan Dung. (2012) Genetic diversity of some edible fungi based on ITS (Internal Transcribed Spacer) sequences. Science Journal of Can Tho University. 22b: 18-25.<\/p>\n\n\n\n<p>Nguyen Loc Hien, Pham Quoc Duy, Huynh Ky and Huynh Thanh Tung. (2012) Genetic diversity of the population of mangosteen (Garcinia mangostana L.) in Lai Thieu (Binh Duong). CAAB Agricultural Science Conference.<\/p>\n\n\n\n<p>Tran Nhan Dung and Do Tan Khang (2012) Genetic diversity of mango varieties (Mangifera sp.) by molecular biology techniques. Science magazine. 22a: 175\u2013185.<\/p>\n\n\n\n<p>Tran Nhan Dung and Tran Thi Le Quyen (2012) Genetic diversity of mangosteen varieties\/lines (Garcinia mangostana L.) based on ISSR molecular markers in Binh Duong. Science Journal of Can Tho University. 23a: 253-261.<\/p>\n\n\n\n<p class=\"has-text-align-right\"><strong>Nguyen Thi Van<\/strong><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Regarding DNA analysis of fruit trees, over the years, there have been quite a few studies on genetic diversity of fruit trees in Vietnam, especially in the Southern region, the typical publications are as follows: Nguyen Loc Hien and ctv. (2012) conducted a study to evaluate genetic diversity through morphology and DNA molecular markers on [&hellip;]<\/p>\n","protected":false},"author":25,"featured_media":30554,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[63],"tags":[],"class_list":["post-30611","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-activity"],"_links":{"self":[{"href":"https:\/\/casta.ttud.com.vn\/index.php\/wp-json\/wp\/v2\/posts\/30611","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/casta.ttud.com.vn\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/casta.ttud.com.vn\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/casta.ttud.com.vn\/index.php\/wp-json\/wp\/v2\/users\/25"}],"replies":[{"embeddable":true,"href":"https:\/\/casta.ttud.com.vn\/index.php\/wp-json\/wp\/v2\/comments?post=30611"}],"version-history":[{"count":1,"href":"https:\/\/casta.ttud.com.vn\/index.php\/wp-json\/wp\/v2\/posts\/30611\/revisions"}],"predecessor-version":[{"id":30612,"href":"https:\/\/casta.ttud.com.vn\/index.php\/wp-json\/wp\/v2\/posts\/30611\/revisions\/30612"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/casta.ttud.com.vn\/index.php\/wp-json\/wp\/v2\/media\/30554"}],"wp:attachment":[{"href":"https:\/\/casta.ttud.com.vn\/index.php\/wp-json\/wp\/v2\/media?parent=30611"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/casta.ttud.com.vn\/index.php\/wp-json\/wp\/v2\/categories?post=30611"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/casta.ttud.com.vn\/index.php\/wp-json\/wp\/v2\/tags?post=30611"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}