{"id":27457,"date":"2020-02-05T15:49:00","date_gmt":"2020-02-05T08:49:00","guid":{"rendered":"https:\/\/ttud.com.vn\/?p=27457"},"modified":"2021-11-06T22:51:17","modified_gmt":"2021-11-06T15:51:17","slug":"quy-trinh-san-xuat-que-thu-phat-hien-nhanh-virut-rota","status":"publish","type":"post","link":"https:\/\/casta.ttud.com.vn\/index.php\/quy-trinh-san-xuat-que-thu-phat-hien-nhanh-virut-rota\/","title":{"rendered":"Quy tr\u00ecnh s\u1ea3n xu\u1ea5t que th\u1eed ph\u00e1t hi\u1ec7n nhanh virut rota"},"content":{"rendered":"\n

Que th\u1eed theo gi\u1ea3i ph\u00e1p n\u00e0y ho\u1ea1t \u0111\u1ed9ng tr\u00ean nguy\u00ean t\u1eafc s\u1eafc k\u00fd mi\u1ec5n d\u1ecbch ch\u1ee9a hai kh\u00e1ng th\u1ec3 \u0111a d\u00f2ng kh\u00e1ng virut rota \u0111\u01b0\u1ee3c s\u1ea3n xu\u1ea5t tr\u00ean hai \u0111\u1ed9ng v\u1eadt kh\u00e1c nhau.<\/strong><\/p>\n\n\n\n

Gi\u1ea3i ph\u00e1p h\u1eefu \u00edch c\u1ee7a nh\u00f3m c\u00e1c nh\u00e0 khoa h\u1ecdc thu\u1ed9c Tr\u01b0\u1eddng \u0110\u1ea1i h\u1ecdc B\u00e1ch Khoa H\u00e0 N\u1ed9i c\u00f3 th\u1ec3 gi\u00fap s\u1ea3n xu\u1ea5t que th\u1eed ph\u00e1t hi\u1ec7n nhanh virut rota \u2013 nguy\u00ean nh\u00e2n g\u00e2y t\u1eed vong do ti\u00eau ch\u1ea3y l\u1edbn nh\u1ea5t \u1edf tr\u1ebb em d\u01b0\u1edbi 5 tu\u1ed5i.<\/p>\n\n\n\n

Theo th\u1ed1ng k\u00ea, h\u1eb1ng n\u0103m c\u00f3 kho\u1ea3ng 25 tri\u1ec7u ng\u01b0\u1eddi m\u1eafc virut rota, trong \u0111\u00f3 c\u00f3 2 tri\u1ec7u ng\u01b0\u1eddi ph\u1ea3i nh\u1eadp vi\u1ec7n. Trong s\u1ed1 600.000 tr\u01b0\u1eddng h\u1ee3p tr\u1ebb t\u1eed vong do virut rota, c\u00f3 h\u01a1n 80% \u0111\u1ebfn t\u1eeb c\u00e1c n\u01b0\u1edbc \u0111ang ph\u00e1t tri\u1ec3n. T\u1eeb n\u0103m 1999, t\u1ea1i Ch\u00e2u \u00c1, h\u1ec7 th\u1ed1ng gi\u00e1m s\u00e1t b\u1ec7nh ti\u00eau ch\u1ea3y do virut rota \u0111\u00e3 \u0111\u01b0\u1ee3c thi\u1ebft l\u1eadp, trong \u0111\u00f3 c\u00f3 s\u1ef1 tham gia c\u1ee7a Vi\u1ec7t Nam. K\u1ebft qu\u1ea3 cho th\u1ea5y t\u1ef7 l\u1ec7 ti\u00eau ch\u1ea3y do virut rota chi\u1ebfm 55-60% tr\u01b0\u1eddng h\u1ee3p. Theo \u01b0\u1edbc t\u00ednh c\u1ee7a T\u1ed5 ch\u1ee9c Y t\u1ebf Th\u1ebf gi\u1edbi, virut rota chi\u1ebfm 4-8% s\u1ed1 ca t\u1eed vong c\u1ee7a tr\u1ebb em d\u01b0\u1edbi 5 tu\u1ed5i. Ti\u00eau ch\u1ea3y do virut rota \u1edf tr\u1ebb em th\u1ef1c s\u1ef1 l\u00e0 b\u00e0i to\u00e1n kh\u00f3 \u0111\u1ed1i v\u1edbi t\u1ea5t c\u1ea3 c\u00e1c n\u01b0\u1edbc tr\u00ean th\u1ebf gi\u1edbi. Ngay c\u1ea3 vi\u1ec7c cung c\u1ea5p n\u01b0\u1edbc s\u1ea1ch v\u00e0 c\u1ea3i thi\u1ec7n \u0111i\u1ec1u ki\u1ec7n v\u1ec7 sinh c\u0169ng kh\u00f4ng gi\u00fap gi\u1ea3m thi\u1ec3u s\u1ed1 tr\u01b0\u1eddng h\u1ee3p b\u1ecb ti\u00eau ch\u1ea3y do virut n\u00e0y.<\/p>\n\n\n\n

B\u00ean c\u1ea1nh vi\u1ec7c gi\u00e1m s\u00e1t d\u1ecbch t\u1ec5 nh\u1eb1m t\u1ea1o ra m\u1ed9t lo\u1ea1i vaccine hi\u1ec7u qu\u1ea3 th\u00ec vi\u1ec7c ch\u1ea9n \u0111o\u00e1n b\u1ec7nh nh\u00e2n ti\u00eau ch\u1ea3y do virut rota g\u00e2y ra l\u00e0 v\u00f4 c\u00f9ng c\u1ea7n thi\u1ebft. M\u1ed9t khi \u0111\u01b0\u1ee3c ch\u1ea9n \u0111o\u00e1n ch\u00ednh x\u00e1c, c\u00e1c b\u00e1c s\u0129 m\u1edbi c\u00f3 th\u1ec3 \u00e1p d\u1ee5ng ph\u01b0\u01a1ng ph\u00e1p \u0111i\u1ec1u tr\u1ecb th\u00edch h\u1ee3p v\u00e0 hi\u1ec7u qu\u1ea3 nh\u1ea5t.<\/p>\n\n\n\n

Hi\u1ec7n nay tr\u00ean th\u1ebf gi\u1edbi \u0111\u00e3 xu\u1ea5t hi\u1ec7n nhi\u1ec1u ph\u01b0\u01a1ng ph\u00e1p ph\u00e1t hi\u1ec7n s\u1ef1 c\u00f3 m\u1eb7t c\u1ee7a virut rota: k\u00ednh hi\u1ec3n vi \u0111i\u1ec7n t\u1eed, ph\u00e1t hi\u1ec7n kh\u00e1ng nguy\u00ean (mi\u1ec5n d\u1ecbch enzyme, ng\u01b0ng k\u1ebft latex v\u00e0 s\u1eafc k\u00fd mi\u1ec5n d\u1ecbch), ph\u00e1t hi\u1ec7n axit nucleic (\u0111i\u1ec7n di polyacrylamit \u2013 PAGE, khu\u1ebfch \u0111\u1ea1i axit nucleic RT-PCR, gi\u1ea3i tr\u00ecnh t\u1ef1 gene). Ph\u1ea7n l\u1edbn c\u00e1c ph\u01b0\u01a1ng ph\u00e1p n\u00e0y \u0111\u1ec1u \u0111\u01b0\u1ee3c WHO khuy\u1ebfn c\u00e1o s\u1eed d\u1ee5ng trong nh\u1eefng tr\u01b0\u1eddng h\u1ee3p nh\u1ea5t \u0111\u1ecbnh, trong \u0111\u00f3 2 ph\u01b0\u01a1ng ph\u00e1p \u0111\u01b0\u1ee3c khuy\u1ebfn c\u00e1o s\u1eed d\u1ee5ng trong x\u00e9t nghi\u1ec7m t\u1ea1i c\u00e1c c\u01a1 s\u1edf y t\u1ebf \u0111\u00f3 l\u00e0 ELISA v\u00e0 s\u1eafc k\u00fd mi\u1ec5n d\u1ecbch (que th\u1eed). Tuy nhi\u00ean, c\u00e1c ph\u01b0\u01a1ng ph\u00e1p n\u00e0y l\u1ea1i t\u01b0\u01a1ng \u0111\u1ed1i kh\u00f3, m\u1ea5t nhi\u1ec1u th\u1eddi gian, \u0111\u00f2i h\u1ecfi c\u00e1c thi\u1ebft b\u1ecb chuy\u00ean d\u1ee5ng v\u00e0 c\u00e1c nh\u00e2n vi\u00ean ph\u1ea3i \u0111\u01b0\u1ee3c \u0111\u00e0o t\u1ea1o chuy\u00ean s\u00e2u, nguy\u00ean v\u1eadt li\u1ec7u t\u01b0\u01a1ng \u0111\u1ed1i \u0111\u1eaft, que th\u1eed ho\u00e0n to\u00e0n ph\u1ea3i nh\u1eadp ngo\u1ea1i. C\u00e1c trung t\u00e2m y t\u1ebf r\u01a1i v\u00e0o t\u00ecnh tr\u1ea1ng kh\u00f4ng ch\u1ee7 \u0111\u1ed9ng \u0111\u01b0\u1ee3c ngu\u1ed3n sinh ph\u1ea9m, \u0111\u1eb7c bi\u1ec7t l\u00e0 thi\u1ebft b\u1ecb nh\u1eadp ngo\u1ea1i c\u00f3 th\u1ec3 kh\u00f4ng ph\u00f9 h\u1ee3p v\u1edbi ch\u1ee7ng virut rota hi\u1ec7n \u0111ang l\u01b0u h\u00e0nh t\u1ea1i Vi\u1ec7t Nam.<\/p>\n\n\n\n

V\u1edbi mong mu\u1ed1n t\u1ea1o ra que th\u1eed ph\u00e1t hi\u1ec7n nhanh virut rota nh\u1eb1m kh\u1eafc ph\u1ee5c c\u00e1c nh\u01b0\u1ee3c \u0111i\u1ec3m tr\u00ean, PGS.TS Tr\u01b0\u01a1ng Qu\u1ed1c Phong v\u00e0 c\u00e1c nh\u00e0 khoa h\u1ecdc thu\u1ed9c Trung t\u00e2m Nghi\u00ean c\u1ee9u v\u00e0 Ph\u00e1t tri\u1ec3n C\u00f4ng ngh\u1ec7 Sinh h\u1ecdc, Vi\u1ec7n C\u00f4ng ngh\u1ec7 Sinh h\u1ecdc v\u00e0 C\u00f4ng ngh\u1ec7 Th\u1ef1c ph\u1ea9m (Tr\u01b0\u1eddng \u0110\u1ea1i h\u1ecdc B\u00e1ch Khoa H\u00e0 N\u1ed9i) \u0111\u00e3 \u0111\u1ec1 xu\u1ea5t m\u1ed9t quy tr\u00ecnh s\u1ea3n xu\u1ea5t que th\u1eed ph\u00e1t hi\u1ec7n nhanh virut rota c\u00f3 gi\u00e1 th\u00e0nh th\u1ea5p, \u0111\u1ed9 nh\u1ea1y v\u00e0 \u0111\u1ed9 \u0111\u1eb7c hi\u1ec7u cao, ph\u00f9 h\u1ee3p v\u1edbi vi\u1ec7c ch\u1ea9n \u0111o\u00e1n c\u00e1c ch\u1ee7ng virut rota l\u01b0u h\u00e0nh t\u1ea1i Vi\u1ec7t Nam.<\/p>\n\n\n\n

Que th\u1eed theo gi\u1ea3i ph\u00e1p n\u00e0y ho\u1ea1t \u0111\u1ed9ng tr\u00ean nguy\u00ean t\u1eafc s\u1eafc k\u00fd mi\u1ec5n d\u1ecbch ch\u1ee9a hai lang th\u1ec3 \u0111a d\u00f2ng kh\u00e1ng virut rota \u0111\u01b0\u1ee3c s\u1ea3n xu\u1ea5t tr\u00ean hai \u0111\u1ed9ng v\u1eadt kh\u00e1c nhau l\u00e0 th\u1ecf v\u00e0 chu\u1ed9t lang, c\u00f9ng m\u1ed9t kh\u00e1ng th\u1ec3 \u0111a d\u00f2ng d\u00ea kh\u00e1ng IgG th\u1ecf. Quy tr\u00ecnh n\u00e0y bao g\u1ed3m c\u00e1c b\u01b0\u1edbc: i) chu\u1ea9n b\u1ecb m\u1eabu huy\u1ebft thanh t\u1eeb th\u1ecf v\u00e0 chu\u1ed9t lang \u0111\u00e3 \u0111\u01b0\u1ee3c g\u00e2y mi\u1ec5n d\u1ecbch b\u1edfi virut rota to\u00e0n ph\u1ea7n; ii) tinh s\u1ea1ch kh\u00e1ng th\u1ec3 \u0111a d\u00f2ng th\u1ecf v\u00e0 chu\u1ed9t lang t\u1eeb m\u1eabu huy\u1ebft thanh thu \u0111\u01b0\u1ee3c \u1edf b\u01b0\u1edbc (i) \u0111\u1ec3 thu \u0111\u01b0\u1ee3c dung d\u1ecbch ch\u1ee9a kh\u00e1ng th\u1ec3 \u0111\u00e3 tinh s\u1ea1ch; iii) c\u00f4 \u0111\u1eb7c dung d\u1ecbch ch\u1ee9a kh\u00e1ng th\u1ec3 \u0111\u00e3 \u0111\u01b0\u1ee3c tinh s\u1ea1ch thu \u0111\u01b0\u1ee3c \u1edf b\u01b0\u1edbc (ii); iv) \u0111i\u1ec1u ch\u1ebf dung d\u1ecbch nano v\u00e0ng; v) t\u1ea1o dung d\u1ecbch c\u1ed9ng h\u1ee3p kh\u00e1ng th\u1ec3 \u0111a d\u00f2ng th\u1ecf kh\u00e1ng virut rota v\u00e0 h\u1ea1t nano v\u00e0ng; vi) t\u1ea1o mi\u1ebfng th\u1ea5m c\u1ed9ng h\u1ee3p b\u1eb1ng c\u00e1ch ng\u00e2m mi\u1ebfng th\u1ea5m trong dung d\u1ecbch c\u1ed9ng h\u1ee3p (v) v\u00e0 s\u1ea5y kh\u00f4; vii) c\u1ed1 \u0111\u1ecbnh kh\u00e1ng th\u1ec3 l\u00ean m\u00e0ng nitroxenluloza; v\u00e0 viii) t\u1ea1o que th\u1eed ph\u00e1t hi\u1ec7n nhanh virut rota.<\/p>\n\n\n\n

\u0110\u1ec3 s\u1eed d\u1ee5ng, ng\u01b0\u1eddi d\u00f9ng s\u1ebd \u0111\u1eb7t que th\u1eed n\u1eb1m ngang, nh\u1ecf 0,1 ml m\u1eabu (3-5 gi\u1ecdt) v\u00e0o v\u1ecb tr\u00ed n\u1ea1p m\u1eabu \u0111\u01b0\u1ee3c \u0111\u00e1nh d\u1ea5u tr\u00ean que th\u1eed, quan s\u00e1t v\u00e0 \u0111\u1ecdc k\u1ebft qu\u1ea3 sau 3-5 ph\u00fat. Khi xu\u1ea5t hi\u1ec7n c\u1ea3 2 v\u1ea1ch h\u1ed3ng t\u00eda \u1edf v\u1ea1ch ki\u1ec3m tra v\u00e0 v\u1ea1ch ki\u1ec3m ch\u1ee9ng, ch\u1ee9ng t\u1ecf m\u1eabu d\u01b0\u01a1ng t\u00ednh v\u1edbi virut rota. Khi ch\u1ec9 c\u00f3 m\u1ed9t v\u1ea1ch h\u1ed3ng t\u00eda \u1edf v\u1ecb tr\u00ed \u0111\u1ed1i ch\u1ee9ng, k\u1ebft lu\u1eadn m\u1eabu \u00e2m t\u00ednh v\u1edbi virut rota.<\/p>\n\n\n\n

Que th\u1eed \u0111\u01b0\u1ee3c t\u1ea1o ra theo quy tr\u00ecnh n\u00e0y c\u00f3 kh\u1ea3 n\u0103ng ph\u00e1t hi\u1ec7n nhanh (3-5 ph\u00fat) virut rota trong m\u1eabu ph\u00e2n b\u1ec7nh ph\u1ea9m. \u0110\u1ed9 nh\u1ea1y v\u00e0 \u0111\u1ed9 \u0111\u1eb7c hi\u1ec7u \u0111\u1ea1t 100%, cao h\u01a1n so v\u1edbi c\u00e1c kit th\u01b0\u01a1ng m\u1ea1i hi\u1ec7n nay. Ng\u01b0\u1ee1ng ph\u00e1t hi\u1ec7n l\u00e0 1,6 x 104<\/sup> h\u1ea1t virut\/ml. Do s\u1eed d\u1ee5ng ngu\u1ed3n kh\u00e1ng th\u1ec3 t\u1ef1 s\u1ea3n xu\u1ea5t n\u00ean c\u00f3 th\u1ec3 ch\u1ee7 \u0111\u1ed9ng \u0111\u01b0\u1ee3c ngu\u1ed3n sinh ph\u1ea9m, \u0111\u1ed3ng th\u1eddi gi\u00e1 th\u00e0nh th\u1ea5p h\u01a1n c\u00e1c kit nh\u1eadp ngo\u1ea1i \u0111ang l\u01b0u h\u00e0nh tr\u00ean th\u1ecb tr\u01b0\u1eddng, n\u00ean c\u00f3 th\u1ec3 d\u1ec5 d\u00e0ng \u00e1p d\u1ee5ng trong th\u1ef1c t\u1ebf.<\/p>\n\n\n\n

V\u1edbi nh\u1eefng \u01b0u \u0111i\u1ec3m \u0111\u00f3, nghi\u00ean c\u1ee9u n\u00e0y m\u1edbi \u0111\u00e2y \u0111\u00e3 \u0111\u01b0\u1ee3c C\u1ee5c S\u1edf h\u1eefu tr\u00ed tu\u1ec7 c\u1ea5p b\u1eb1ng \u0111\u1ed9c quy\u1ec1n gi\u1ea3i ph\u00e1p h\u1eefu \u00edch s\u1ed1 2-0002106 c\u00f4ng b\u1ed1 ng\u00e0y 25\/9\/2019. \u0110\u00e2y l\u00e0 m\u1ed9t \u0111i\u1ec1u v\u00f4 c\u00f9ng k\u1ecbp th\u1eddi, khi m\u00e0 theo C\u1ee5c Y t\u1ebf d\u1ef1 ph\u00f2ng (B\u1ed9 Y t\u1ebf), m\u00f9a \u0110\u00f4ng – Xu\u00e2n, v\u1edbi \u0111i\u1ec1u ki\u1ec7n th\u1eddi ti\u1ebft \u1ea9m \u01b0\u1edbt, nh\u1ea5t l\u00e0 s\u1ef1 gia t\u0103ng trong giao l\u01b0u \u0111i l\u1ea1i v\u00e0o d\u1ecbp T\u1ebft Nguy\u00ean \u0111\u00e1n v\u00e0 l\u1ec5 h\u1ed9i m\u00f9a xu\u00e2n s\u1ebd l\u00e0 \u0111i\u1ec1u ki\u1ec7n thu\u1eadn l\u1ee3i cho c\u00e1c b\u1ec7nh truy\u1ec1n nhi\u1ec5m l\u00e2y lan, b\u00f9ng ph\u00e1t, \u0111\u1eb7c bi\u1ec7t l\u00e0 b\u1ec7nh ti\u00eau ch\u1ea3y do virut rota g\u00e2y ra.<\/p>\n\n\n\n

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Th\u1eed s\u1ea3n ph\u1ea9m que th\u1eed ph\u00e1t hi\u1ec7n nhanh virut\u00a0rota g\u00e2y b\u1ec7nh ti\u00eau ch\u1ea3y \u1edf tr\u1ebb em t\u1ea1i Vi\u1ec7n C\u00f4ng ngh\u1ec7 sinh h\u1ecdc v\u00e0 C\u00f4ng ngh\u1ec7 th\u1ef1c ph\u1ea9m, \u0110\u1ea1i h\u1ecdc B\u00e1ch khoa H\u00e0 N\u1ed9i. \u1ea2nh: Ho\u00e0ng Hi\u1ec7p<\/em><\/figcaption><\/figure><\/div>\n\n\n\n

<\/p>\n\n\n\n

Ngu\u1ed3n: Anh Th\u01b0- https:\/\/www.ipviet<\/em>nam.gov.vn\/tin-tuc-su-kien\/-\/asset_publisher\/7xsjBfqhCDAV\/content\/quy-trinh-san-xuat-que-thu-phat-hien-nhanh-virus-rota<\/em><\/p>\n","protected":false},"excerpt":{"rendered":"

Que th\u1eed theo gi\u1ea3i ph\u00e1p n\u00e0y ho\u1ea1t \u0111\u1ed9ng tr\u00ean nguy\u00ean t\u1eafc s\u1eafc k\u00fd mi\u1ec5n d\u1ecbch ch\u1ee9a hai kh\u00e1ng th\u1ec3 \u0111a d\u00f2ng kh\u00e1ng virut rota \u0111\u01b0\u1ee3c s\u1ea3n xu\u1ea5t tr\u00ean hai \u0111\u1ed9ng v\u1eadt kh\u00e1c nhau. Gi\u1ea3i ph\u00e1p h\u1eefu \u00edch c\u1ee7a nh\u00f3m c\u00e1c nh\u00e0 khoa h\u1ecdc thu\u1ed9c Tr\u01b0\u1eddng \u0110\u1ea1i h\u1ecdc B\u00e1ch Khoa H\u00e0 N\u1ed9i c\u00f3 th\u1ec3 gi\u00fap s\u1ea3n […]<\/p>\n","protected":false},"author":25,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[47],"tags":[],"class_list":["post-27457","post","type-post","status-publish","format-standard","hentry","category-tin-tuc"],"_links":{"self":[{"href":"https:\/\/casta.ttud.com.vn\/index.php\/wp-json\/wp\/v2\/posts\/27457","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/casta.ttud.com.vn\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/casta.ttud.com.vn\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/casta.ttud.com.vn\/index.php\/wp-json\/wp\/v2\/users\/25"}],"replies":[{"embeddable":true,"href":"https:\/\/casta.ttud.com.vn\/index.php\/wp-json\/wp\/v2\/comments?post=27457"}],"version-history":[{"count":2,"href":"https:\/\/casta.ttud.com.vn\/index.php\/wp-json\/wp\/v2\/posts\/27457\/revisions"}],"predecessor-version":[{"id":29536,"href":"https:\/\/casta.ttud.com.vn\/index.php\/wp-json\/wp\/v2\/posts\/27457\/revisions\/29536"}],"wp:attachment":[{"href":"https:\/\/casta.ttud.com.vn\/index.php\/wp-json\/wp\/v2\/media?parent=27457"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/casta.ttud.com.vn\/index.php\/wp-json\/wp\/v2\/categories?post=27457"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/casta.ttud.com.vn\/index.php\/wp-json\/wp\/v2\/tags?post=27457"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}