{"id":26873,"date":"2021-06-04T08:46:24","date_gmt":"2021-06-04T01:46:24","guid":{"rendered":"http:\/\/ttud.com.vn\/?p=26873"},"modified":"2021-06-04T08:46:29","modified_gmt":"2021-06-04T01:46:29","slug":"phuong-phap-moi-de-san-xuat-adn-tong-hop","status":"publish","type":"post","link":"https:\/\/casta.ttud.com.vn\/index.php\/phuong-phap-moi-de-san-xuat-adn-tong-hop\/","title":{"rendered":"Ph\u01b0\u01a1ng ph\u00e1p m\u1edbi \u0111\u1ec3 s\u1ea3n xu\u1ea5t ADN t\u1ed5ng h\u1ee3p"},"content":{"rendered":"\n<h2 class=\"wp-block-heading\"><strong>C\u00e1c nh\u00e0 khoa h\u1ecdc t\u1ea1i \u0110\u1ea1i h\u1ecdc Aarhus, \u0110an M\u1ea1ch, \u0111\u00e3 ph\u00e1t tri\u1ec3n m\u1ed9t c\u00f4ng ngh\u1ec7 t\u01b0\u01a1ng \u0111\u1ed1i \u0111\u01a1n gi\u1ea3n nh\u01b0ng hi\u1ec7u qu\u1ea3, trong \u0111\u00f3 vi\u1ec7c s\u1ea3n xu\u1ea5t photphoramidit c\u00f3 th\u1ec3 \u0111\u01b0\u1ee3c t\u1ef1 \u0111\u1ed9ng h\u00f3a v\u00e0 t\u00edch h\u1ee3p tr\u1ef1c ti\u1ebfp v\u00e0o thi\u1ebft b\u1ecb t\u1ed5ng h\u1ee3p ADN.<\/strong><\/h2>\n\n\n\n<p>C\u00e1c chu\u1ed7i ADN ng\u1eafn \u0111\u01b0\u1ee3c t\u1ed5ng h\u1ee3p h\u00f3a h\u1ecdc l\u00e0 th\u00e0nh ph\u1ea7n c\u1ef1c k\u1ef3 quan tr\u1ecdng v\u1edbi v\u00f4 s\u1ed1 \u1ee9ng d\u1ee5ng trong c\u00e1c ph\u00f2ng th\u00ed nghi\u1ec7m, b\u1ec7nh vi\u1ec7n v\u00e0 trong c\u00f4ng nghi\u1ec7p. Tr\u00ecnh t\u1ef1 ADN \u0111\u01b0\u1ee3c t\u1ea1o ra c\u00f2n \u0111\u01b0\u1ee3c g\u1ecdi l\u00e0 oligonucleotide. Ch\u00fang \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng r\u1ed9ng r\u00e3i \u0111\u1ec3 x\u00e1c \u0111\u1ecbnh b\u1ec7nh t\u1eadt, s\u1ea3n xu\u1ea5t thu\u1ed1c d\u1ef1a tr\u00ean oligonucleotide&nbsp;v\u00e0 cho m\u1ed9t s\u1ed1 \u1ee9ng d\u1ee5ng y t\u1ebf v\u00e0 c\u00f4ng ngh\u1ec7 sinh h\u1ecdc kh\u00e1c.<\/p>\n\n\n\n<p>Do \u0111\u00f3, nhu c\u1ea7u cao v\u1ec1 oligonucleotide \u0111\u00f2i h\u1ecfi m\u1ed9t ph\u01b0\u01a1ng ph\u00e1p t\u1ef1 \u0111\u1ed9ng h\u00f3a hi\u1ec7u qu\u1ea3 \u0111\u1ec3 s\u1ea3n xu\u1ea5t h\u00f3a ch\u1ea5t c\u1ee7a ch\u00fang. Qu\u00e1 tr\u00ecnh n\u00e0y d\u1ef1a v\u00e0o phosphoramidit, l\u00e0 nh\u1eefng h\u1ee3p ch\u1ea5t h\u00f3a h\u1ecdc c\u00f3 nh\u01b0\u1ee3c \u0111i\u1ec3m l\u00e0 kh\u00f4ng \u1ed5n \u0111\u1ecbnh tr\u1eeb khi \u0111\u01b0\u1ee3c b\u1ea3o qu\u1ea3n \u1edf nhi\u1ec7t \u0111\u1ed9 -20 \u0111\u1ed9 C l\u00fd t\u01b0\u1edfng. C\u00e1c thi\u1ebft b\u1ecb \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng \u0111\u1ec3 t\u1ed5ng h\u1ee3p ADN kh\u00f4ng th\u1ec3 l\u00e0m ngu\u1ed9i c\u00e1c phosphoramidit, v\u00e0 do \u0111\u00f3, kh\u00f4ng th\u1ec3 tr\u00e1nh kh\u1ecfi vi\u1ec7c m\u1ed9t s\u1ed1 ch\u00fang b\u1ecb ph\u00e2n hu\u1ef7 sau khi \u0111\u01b0\u1ee3c th\u00eam v\u00e0o thi\u1ebft b\u1ecb.<\/p>\n\n\n\n<p>C\u00e1c nh\u00e0 khoa h\u1ecdc t\u1ea1i \u0110\u1ea1i h\u1ecdc Aarhus, \u0110an M\u1ea1ch, \u0111\u00e3 ph\u00e1t tri\u1ec3n m\u1ed9t c\u00f4ng ngh\u1ec7 t\u01b0\u01a1ng \u0111\u1ed1i \u0111\u01a1n gi\u1ea3n nh\u01b0ng hi\u1ec7u qu\u1ea3, trong \u0111\u00f3 vi\u1ec7c s\u1ea3n xu\u1ea5t photphoramidit c\u00f3 th\u1ec3 \u0111\u01b0\u1ee3c t\u1ef1 \u0111\u1ed9ng h\u00f3a v\u00e0 t\u00edch h\u1ee3p tr\u1ef1c ti\u1ebfp v\u00e0o thi\u1ebft b\u1ecb t\u1ed5ng h\u1ee3p ADN. \u0110i\u1ec1u n\u00e0y tr\u00e1nh \u0111\u01b0\u1ee3c c\u1ea3 vi\u1ec7c t\u1ed5ng h\u1ee3p th\u1ee7 c\u00f4ng c\u00e1c h\u1ee3p ch\u1ea5t n\u00e0y, th\u01b0\u1eddng m\u1ea5t \u0111\u1ebfn 12 gi\u1edd, c\u0169ng nh\u01b0 v\u1ea5n \u0111\u1ec1 l\u01b0u tr\u1eef c\u00e1c phosphoramidit kh\u00f4ng \u1ed5n \u0111\u1ecbnh.&nbsp;<\/p>\n\n\n\n<p>Ph\u01b0\u01a1ng ph\u00e1p s\u1ea3n xu\u1ea5t m\u1edbi n\u00e0y tr\u00e1nh \u0111\u01b0\u1ee3c s\u1ef1 xu\u1ed1ng c\u1ea5p c\u1ee7a c\u00e1c phosphoramidit, v\u00ec ch\u00fang \u0111\u01b0\u1ee3c s\u1ea3n xu\u1ea5t ngay tr\u01b0\u1edbc khi \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng (theo y\u00eau c\u1ea7u), theo c\u00e1ch nhanh h\u01a1n, hi\u1ec7u qu\u1ea3 h\u01a1n.<\/p>\n\n\n\n<p>                                                                                      ngu\u1ed3n: khoahocdoisong.vn<\/p>\n","protected":false},"excerpt":{"rendered":"<p>C\u00e1c nh\u00e0 khoa h\u1ecdc t\u1ea1i \u0110\u1ea1i h\u1ecdc Aarhus, \u0110an M\u1ea1ch, \u0111\u00e3 ph\u00e1t tri\u1ec3n m\u1ed9t c\u00f4ng ngh\u1ec7 t\u01b0\u01a1ng \u0111\u1ed1i \u0111\u01a1n gi\u1ea3n nh\u01b0ng hi\u1ec7u qu\u1ea3, trong \u0111\u00f3 vi\u1ec7c s\u1ea3n xu\u1ea5t photphoramidit c\u00f3 th\u1ec3 \u0111\u01b0\u1ee3c t\u1ef1 \u0111\u1ed9ng h\u00f3a v\u00e0 t\u00edch h\u1ee3p tr\u1ef1c ti\u1ebfp v\u00e0o thi\u1ebft b\u1ecb t\u1ed5ng h\u1ee3p ADN. C\u00e1c chu\u1ed7i ADN ng\u1eafn \u0111\u01b0\u1ee3c t\u1ed5ng h\u1ee3p h\u00f3a h\u1ecdc [&hellip;]<\/p>\n","protected":false},"author":23,"featured_media":26874,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[47],"tags":[],"class_list":["post-26873","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-tin-tuc"],"_links":{"self":[{"href":"https:\/\/casta.ttud.com.vn\/index.php\/wp-json\/wp\/v2\/posts\/26873","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/casta.ttud.com.vn\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/casta.ttud.com.vn\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/casta.ttud.com.vn\/index.php\/wp-json\/wp\/v2\/users\/23"}],"replies":[{"embeddable":true,"href":"https:\/\/casta.ttud.com.vn\/index.php\/wp-json\/wp\/v2\/comments?post=26873"}],"version-history":[{"count":3,"href":"https:\/\/casta.ttud.com.vn\/index.php\/wp-json\/wp\/v2\/posts\/26873\/revisions"}],"predecessor-version":[{"id":26883,"href":"https:\/\/casta.ttud.com.vn\/index.php\/wp-json\/wp\/v2\/posts\/26873\/revisions\/26883"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/casta.ttud.com.vn\/index.php\/wp-json\/wp\/v2\/media\/26874"}],"wp:attachment":[{"href":"https:\/\/casta.ttud.com.vn\/index.php\/wp-json\/wp\/v2\/media?parent=26873"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/casta.ttud.com.vn\/index.php\/wp-json\/wp\/v2\/categories?post=26873"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/casta.ttud.com.vn\/index.php\/wp-json\/wp\/v2\/tags?post=26873"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}